E-钙黏蛋白和β-连环蛋白在Ⅰ型子宫内膜癌中的表达及其意义

The expression and significance of E-cadherin and β-catenin in type Ⅰ endometrial carcinoma

目的 探讨E-钙黏蛋白（E-cadherin）和β-连环蛋白（β-catenin）在Ⅰ型子宫内膜癌中的表达及其临床意义.方法 采用免疫组织化学GT-Vision法检测E-cadherin和β-catenin在60例子宫内膜癌及10例正常增生期子宫内膜组织中的表达情况,术后对患者随访以了解其表达与生存率的关系.结果 正常增生期子宫内膜中E-cadherin和β-catenin均阳性表达,均定位于细胞膜;在Ⅰ型子宫内膜癌中E-cadherin和β-catenin发生异常表达,异常表达率分别为60.0％（36/60）、55.0％（33/60）,差异均有统计学意义（均P＜ 0.001）;有淋巴结转移组中E-cadherin和β-catenin阳位表达率较无淋巴结转移组低（均P＜ 0.05）.Ⅰ型子宫内膜癌E-cadherin和β-catenin的异常表达水平与肿瘤细胞组织学分级、子宫肌层浸润深度呈正相关（均P＜ 0.01）.E-cadherin和β-catenin表达异常者术后2年生存率低于表达正常者（均P＜ 0.05）.结论 E-cadherin和β-catenin异常高表达可能在Ⅰ型子宫内膜癌的发生、转移过程中起重要作用,是判断子宫内膜癌浸润转移及近期预后的良好指标之一.

Objective To investigate the expression and significance of E-cadherin and β-catenin in type Ⅰ endometrial carcinoma.Methods The expression of E-cadherin and β-catenin were detected by immunohistochemistry in 60 patients with type Ⅰ endometrial carcinoma and 10 normal endometrial tissues.The patients were followed-up postoperatively for the survival rate.Results Expression of E-cadherin and β-catenin in 10 normal endometrial tissues was all high expression,while those of both abnormal expression in type Ⅰ endometrial carcinoma with expression rates with 60.0 % （36/60） and 55.0 % （33/60）,respectively,which were difference compared with both in normal endometrial tissues （P 〈 0.05）.The expression rates were markedly decreased in carcinoma with lymph node metastasis than those without lymph node metastasis.The proportions of abnormal expressions of E-cadherin and β-catenin were positively correlated with tumor grade and myometrial invasion in type Ⅰ endometrial carcinoma （P 〈 0.05）.The postoperative two-year survival rate in the patients with abnormal expression of E-cadherin and β-catenin were much lower than those with normal expression （P 〈 0.05）.Conclusions Abnormal high expression of E-cadherin and β-catenin may play important roles on generation and metastasis in type Ⅰ endometrial carcinoma.Both of them may be promising method for predicting invasion and metastasis and can be prognostic predictors in type Ⅰ endometrial carcinoma.