樱花多糖提取工艺的优化及抗氧化性研究

Study on Optimization of Extraction Process of Cerasus Serrulata Polysaccharide and Its Antioxidant Activity

目的:通过正交试验优化樱花多糖的提取工艺,对樱花多糖的抗氧化功能进行了相关的研究,为樱花多糖保健功能的研究提供试验依据。方法:通过正交设计优化樱花多糖的提取工艺,用蒽酮-硫酸法测定不同提取条件下多糖的含量,并对其进行抗氧化性试验和自由基的清除试验。结果与结论:通过对试验结果进行分析,最优工艺为3 g干燥的樱花花瓣置于烧杯中,加蒸馏水240 m L,在90℃的水浴锅中提取3 h,过滤,离心,得上清液。各种浓度的樱花多糖提取液均能够有效地清除超氧阴离子自由基(O2-·),且随着浓度的增大,其清除能力逐渐增强。浓度为70.5μg·L-1多糖提取液的清除率为58.82%。

Objective： To optimize the extraction process of cerasus serrulata polysaccharide by the orthogonal experiment, conduct the related research of antioxidant function of cerasus serrulata polysaccharide and provide experimental basis for the research on the healthcare function of cerasus serrulata polysaccharide. Methods： The orthogonal design was used to optimize the extraction process of polysaccharide. The anthrone-sulfuric acid method was used to determine the content of polysaccharides in different extraction conditions and the oxidation resistance test and the free-radical clearance test were conducted. Results and Conclusion： Through the analysis of the test results, the optimum process was as follows. The 3 g dried Cerasus serrulata petals were put in the beaker with 240 m L distillated water and were extracted for 3 hours in the 90 ℃ water bath pot to obtain the supernatant through filtering and centrifugation. Cerasus serrulata polysaccharide extracts of various concentrations could effectively remove superoxide anion free radicals（O2-·）. Along with the increase of the concentration, its removal ability was gradually improved. The clearance rate of the polysaccharide extract at a concentration of 70.5 μg·L-1 was 58.82%.