摘要
粪产碱菌在以阿魏酸为唯一碳源的厌氧发酵中培养7 d,阿魏酸的降解率约为70%;研究秸秆厌氧发酵产气(以产气量反应菌群的活性)发现,体系接种5%粪产碱菌后,菌群的活性最强,生物气增量最大,达130 m L,比接种3%和7%粪产碱菌的体系提高85.71%和116.67%;同时体系的产酸效率和阿魏酸的降解率均显著提升,分别比接种3%和7%粪产碱菌的体系提高136%和110.71%以及25%和33.33%。傅里叶红外光谱检测表明:厌氧发酵体系接种5%粪产碱菌后秸秆中木质素、阿魏酸的特征官能团结构被有效破坏。秸秆厌氧发酵体系接入粪产碱菌可以有效降解阿魏酸等木质素降解衍生物、解除木质素及其降解产物对厌氧菌群的毒性同时提高产气效率,具有应用价值。
The ferulic acid was approximately degradated 70% when Alcaligenes faecalis was cultured 7 d in the anaerobic culture with ferulic acid as the sole carbon source. Anaerobic fermentation systems with straw producing biogas were studied, where the biogas production was used to reflect the bacteria activity. Results showed that biogas production with A. faecalis inoculum 5% was up to 130 ml, which was increased by 85.71% and 116.67% compared with the inoculum of 3% and 7%, respectively. Meanwhile, the acids production, reflected by pH, was enhanced by 136% and 110.71%, and the ferulic acid degradation rate was increased by 25% and 33.33% compared with the inoculum of 3% and 7%, respectively. Fourier infrared spectrum analysis showed that the characteristic structure oflignin and ferulic acid in the anaerobic system with A. faecalis addition 5% was destroyed effectively. The results showed that A. faecalis inoculated in anaerobic fermentation system with straw could efficiently degrade lignin degradation derivatives (such as ferulic acid), remove the toxicity of lignin and its degradation products for the anaerobic bacteria group and enhance biogas production.
出处
《中国酿造》
CAS
北大核心
2015年第3期66-70,共5页
China Brewing
基金
湖北省科技厅项目(2010CDB02505)
关键词
粪产碱菌
厌氧降解
阿魏酸
木质素
Alcaligenes faecalis
anaerobic degradation
ferulic acid
lignin