摘要
背景:不同的神经受体通过不同的受体介导产生各自的生物功能,脊髓损伤的根本原因是神经元的死亡和突触的功能丧失,人身皂苷Rg1对神经元细胞可能具有保护作用。目的:验证人身皂苷Rg1对SD大鼠脊髓神经元细胞的营养保护作用。方法:对孕龄16日的胚鼠进行脊髓神经元细胞分离提取,对神经元细胞进行冻存和复苏。实验分为3组:空白组、人参皂苷Rg1干预组、阳性对照组(培养孔内加入苯酚溶液)。采用MTT法检测细胞内线粒体的活性及人参皂甙Rg1对神经元细胞的增殖作用。结果与结论:人参皂苷Rg1干预组细胞吸光度显著高于空白对照组细胞,人参皂苷Rg1干预组细胞的相对活力高于空白组,说明人参皂苷Rg1对神经元细胞具有促进生长的作用。人参皂苷Rg1干预后,细胞的突起增长速度明显快于空白组的细胞,说明人参皂苷Rg1对脊髓神经元细胞具有保护作用,可抑制神经元细胞凋亡,使细胞活力增强。
BACKGROUND: Different neuroreceptors medicated by different receptors produce their own biological functions. The root cause of spinal cord injury is the death of neurons and the synaptic dysfunction. Ginsenoside Rgl may play a protective role in neurons. OBJECTIVE: To verify the nutritional and protective roles of ginsenoside Rgl in spinal neurons of Sprague-Dawley rats. METHODS: The spinal neurons were isolated from embryonic rats at a gestation age of 16 days, and following cryopreservation and resuscitation, the cells were divided into three groups: blank group, ginsenoside Rgl group and positive control group (phenol solution). MTT method was used to detect the activity of mitochondria and ginsenoside Rgl effects on the neuronal proliferation. RESULTS AND CONCLUSION: Compared with the blank group, the cell absorbance value and cell relative activity were both higher in the ginsenoside Rgl group, indicating that ginsenoside Rgl can encourage the growth of neurons. When cells were intervened by ginsenoside Rgl, cell processes grew faster than those in the blank group, indicating ginsenoside Rgl can protect the neurons, inhibit neuronal apoptosis, and boost the cell vitality.
出处
《中国组织工程研究》
CAS
北大核心
2015年第7期1003-1007,共5页
Chinese Journal of Tissue Engineering Research
基金
上海市宝山区卫生青年医学人才培养计划资助(bswsyq-2014-A03)~~