摘要
目的检测血红素加氧酶-1在豚鼠耳蜗螺旋神经节顺铂损伤后的表达变化。方法通过免疫组织化学及Western Blot技术,观察耳蜗螺旋神经元损伤后HO-1在不同组间的表达变化。将32只健康豚鼠随机分为4组,每组8只。A组对照组:按体重以0.9%生理盐水12ml/kg腹腔注射,早晚各一次,持续3天。B组顺铂组:按体重12mg/kg顺铂腹腔注射(IP),早晚各一次,持续3天。C组阿魏酸(FA)+顺铂组:先150mg/kg FA腹腔注射预处理1h,再以12mg/kg顺铂IP,早晚各一次,持续三天。D组FA+锌原卟啉Ⅸ(ZnppⅨ)+顺铂组:同时给予150mg/kg FA+ZnppⅨ15umol/kg IP预处理1h,再以12mg/kg顺铂IP,早晚各一次,持续三天。采用免疫组织化学及Wstern Blot技术检测不同组间螺旋神经元HO-1蛋白表达变化。结果在不同的分组中,HO-1在耳蜗螺旋神经节中表达量不同。空白组中,螺旋神经节组织存在微弱的HO-1表达,其蛋白表达量为0.48±0.07;A组蛋白表达量为0.81±0.07,B组蛋白表达量为0.9±0.05,C组蛋白表达量为0.61±0.07。4个组两两间差异均有统计学意义(p<0.0001)。结论 HO-1在C组中表达量最多,B组次之,D组最少,表明HO-1可能参与顺铂损伤螺旋神经元后的修复过程。
Objective To investigate effects of heme oxygenase-l（HO-1） on spiral ganglion cells neurons （SGNs） in cis- platin-induced injury. Methods Forty adult guinea pigs were divided randomly into 4 groups （n=8 in each group） to receive intraperitoneal injection of 0.9% saline （Group A）, or cisplatin （Group B）, or ferulic acid （150 mg/kg, bid） and cisplatin （Group C）, or ferulic acid （150 mg/kg, bid）, zinc-protoporphyrin-Ⅸ （15 umol/kg, bid） and cisplatin （Group D）, all at 12 ml/kg, bid for 3 days. HO-1 expressing pattern in damaged cochlea was studied by immunochemistry and Western Blot techniques. Results in Group A, faint HO-1 expression was seen in the spiral ganglion tissue with its protein expression only at 0.48 ± 0.07. HO-1 protein expression was 0.81± 0.07 in Group B, 0.9±0.05 in Group C and 0.61 ± 0.07 in Group D. The differences in HO-1 pro- tein expression between the four groups were statistically significant （p〈0.0001）. Conclusion Among animals exposed to cispl- atin, expression of HO-1 was the highest in Group C and the lowest in Group D, indicating possible HO-1 involvement in the re- pair process in spiral neurons following damage by cisplatin.
出处
《中华耳科学杂志》
CSCD
北大核心
2015年第1期156-160,共5页
Chinese Journal of Otology
基金
重点项目(2012-1-041)
