长寿家系COMT Val158Met基因多态性及其与血压和血脂水平的相关性

Association of COMT Val158Met polymorphism with blood pressure and serum lipid levels in long-lived families

目的探讨长寿家系COMT Val158Met(G>A)基因多态性与血压、血脂水平的相关性,了解其在长寿中的作用。方法用PCR-RFLP方法对广西红水河流域长寿家系(HL)及非长寿家系(HNL)、外地区(平果)长寿(PL)及非长寿家系(PNL)的COMT Val158Met进行基因分型,分析该多态与血压和血脂水平的相关性。结果等位基因A及其基因型(GA/AA)频率在HL和HNL间无差异,但高于PL和PNL(P<0.01)。HL和PNL的血压、血脂水平在GA/AA和GG基因型携带者之间无差异,但HNL的GA/AA携带者的收缩压、脉压差、TC、TG及LDL-C水平明显高于GG携带者(P<0.05),尤以女性为甚,PL的部分结果与此类似。COMT Val158Met多态与HNL及PL的TC和LDL-C水平呈负相关(P<0.05)。结论 COMT Val158Met虽然在红水河流域人群中高频出现,但其对血压及血脂的影响主要在一般家系,对长寿家系的影响较小。

Objective To explore the potential correlation between the COMT Val158Met polymorphism and blood pressure and serum lipid levels in a family-based long-lived population. Methods COMT Val158Met was genotyped with PCR-RFLP for long-lived families （HL） and non-long-lived families （HNL） from Guangxi Hongshuihe River Basin and two controls outside the Basin （Pingguo long-lived families [ PL] and non-long- lived families [ PNL ] ）. The correlations among this polymorphism, blood pressure and lipids were then evalu- ated. Results No difference was found in the frequencies of allele A and its relevant genotypes （GA/AA） between HL and HNL, they however were significantly higher than that of PL and PNL （P 〈 0.01 ）. While the blood pressure and lipid levels were similar between GA/AA and GG in HL and PNL, the systolic pressure,pulse pressure and levels of TC, TG and LDL-C of GA/AA were noted higher than that of GG in HNL. COMT Val158Met was negatively associated with TC and LDL-C levels in HNL and PL. Conclusions COMT 158Met mainly impact on blood pressure in the general families rather than the long-lived families in Guangxi Hongshuihe River Basin.