弥漫大B细胞淋巴瘤患者尿液中挥发性标志物的筛选

The screening of urinary volatile markers for diffuse large B-cell lymphoma

目的筛选弥漫大B细胞淋巴瘤(DLBCL)患者尿液中有诊断价值的挥发性标志物(VOCs)。方法收集35例DLBCL患者尿液样本(DLBCL组),30例健康者尿液样本(对照组)。利用顶空气体固相微萃取联用气相色谱-质谱检测分析两组尿液样本中的挥发性有机化合物。应用Wilcoxon非参数检验分析筛选DLBCL患者尿液中的特异性VOCs。结果筛选出正己烷、2-丁酮和4-庚酮3种特异性挥发性标志物。DLBCL组正己烷的浓度为(63.45±9.98)μg/ml,2-丁酮的浓度为(105.43±12.81)μg/ml,4-庚酮的浓度为(162.56±25.38)μg/ml。3种物质的浓度在DLBCL组和对照组之间差异有统计学意义(P<0.05)。结论 DLBCL患者尿液中挥发性化合物(正己烷、2-丁酮和4-庚酮)可能是有诊断价值的VOCs。

Objective To screen urinary volatile organic compounds（ VOCs） in diffuse large B-cell lymphoma（ DL-BCL） patients . Methods The urine samples were collected from 35 DLBCL patients and 30 healthy controls. VOCs were extracted with head space solid phase micro-extract （ SPME ） combined with the gas chromatography （ GC）-masss pectrometry（ MS） . The non-parametric Wilcoxon test was used for discriminating the VOCs and asses-sing the statistical significance. Results Hexane, 2-butanone and 4-heptanone were found as the specific VOCs for DLBCL. The concentration range of the hexane was（63. 45 ± 9. 98） μg/ml,and the concentration range of 2-buta-none was（105. 43 ± 12. 81） μg/ml. As for the concentration range of 4-heptanone, it was （162. 56 ± 25. 38） μg/ml. The concentration of the three VOCs was significantly different between DLBCL patients and healthy controls（ P〈0. 05）. Conclusion Hexane, 2-butanone and 4-heptanone may be the latent volatile biomarkers for the diagno-sis of patients with DLBCL.