酒精依赖对地西泮抗惊厥作用的影响

Impacts of alcohol dependence on the anticonvulsant effect of diazepam

目的：研究酒精依赖对地西泮抗惊厥作用的影响。方法成年昆明种小鼠36只，均分为酒精依赖组（A组）、地西泮组（D组）和生理盐水组（N组）。A组用含0.8％乙醇的生理盐水进行腹腔注射，其他2组注射生理盐水，每天上、下午各1次，0.2 mL/次，注射7 d。检测小鼠7 d的自主活动情况。7 d后行电惊厥实验，A组和D组腹腔注射0.05%地西泮溶液0.05 mL/10 g，N组注射等体积生理盐水，给药前及给药后15、30、60 min分别测量3组小鼠惊厥阈值。结果第2～6天，A组小鼠自主活动计数小于其他2组（P＜0.05），第1和7天，3组小鼠自主活动计数差异无统计学意义。给药前，A组惊厥阈值高于D组和N组（P＜0.05）；给药后，N组惊厥阈值与给药前相比差异无统计学意义；给药后15 min，D组惊厥阈值高于A组和N组，A组阈值高于N组（P＜0.05）；给药后30 min、60 min，A组和D组惊厥阈值均高于N组（P＜0.05），A组和D组惊厥阈值差异无统计学意义。结论酒精依赖有抗惊厥的作用；酒精依赖会使地西泮的抗惊厥作用减弱。

Objective To study the impacts of alcohol dependence on the anticonvulsant effect of diazepam. Meth？ods Kunming mice （n=36） were divided into 3 groups （n=12 in each group）, Alcohol Dependence Group（A group）, Diaze？pam Group（D group）and Normal Saline Group（N group）. A group received an intraperitoneal injection with a 0.2 mL dose of 0.8%alcohol in NS （normal saline） , while both D and N group received an injection with a 0.2 mL dose of NS without alco？hol , twice a day. Mice’s autonomic activities were monitored every day. After 7 days, the electroconvulsive experiment was performed. Both A and D group were given a weight-based dose of 0.05 mL/10 g of 0.05%diazepam via intraperitoneal injec？ tion, while N group was given a 0.05 mL/10 g dose of NS. Before administration and after 15, 30, 60 min of administration, the convulsion threshold of each group was measured. Results The count of autonomic activity of mice in A group was less than that of mice in D and N group during the 2nd day to 6th day（P〈0.05）. On the 1st and 7th day, the difference of the count of autonomic activity of mice between A group and the other two groups was not statistically significant（P〉0.05）. The convulsion threshold of mice in A group was higher than that of mice in D and N group before administration（P〈0.05）. Af？ter administration, the convulsion threshold of mice in N group didn’t show statistically significant difference from that of mice before administration（P〉0.05）. After 15 min of administration, the convulsion threshold of mice in D group was high？er than that of mice in A and N group（P〈0.05）, while the convulsion threshold of mice in A group was higher than that of mice in N group（P〈0.05）. After 30 min and 60 min of administration, both the convulsion thresholds of mice in A and D group were higher than that of mice in N group（P〈0.05）. However, at this point, the difference of the convulsion thresholds of mice between A and D group was not statistically significant（P〉0.05）. Conclusion Alcohol dependence has anticon？vulsant effect. Alcohol dependence weakens the anticonvulsant effect of diazepam.