Drosha在子宫内膜癌组织中的差异表达及临床意义

Clinical significance of differential Drosha expression between endometrial cancer tissue and other tissues

目的探讨Drosha在子宫内膜癌组织中的表达及临床意义。方法应用实时荧光定量PCR(q RT-PCR)和Western blot检测正常子宫内膜组织(25例)、不典型增生子宫内膜组织(20例)及子宫内膜癌组织(40例)中Drosha的表达,分析Drosha在子宫内膜癌组织中的表达与临床病理因素之间的关系。结果子宫内膜癌组Drosham RNA及蛋白表达水平均低于不典型增生子宫内膜组及正常子宫内膜组(均P<0.05),不典型增生子宫内膜组及正常子宫内膜组的表达差异无统计学意义(均P>0.05)。不同年龄、组织分级、病理分型、淋巴结转移、肌层浸润深度和FIGO分期的子宫内膜癌患者的Drosha m RNA表达差异无统计学意义(均P>0.05)。结论 Drosha在子宫内膜癌组织中表达下调,可能与子宫内膜癌的发生发展有关。

Objective To detect the different expressions of Drosha between endometrial cancer （EC） tissue and other tissues and to explore correlation between Drosha mRNA transcription level and protein expression level with clinicopatho？logical characteristics of EC. Methods The mRNA transcription and protein expression levels of Drosha were examinaned by q-PCR and Western blot respectively in normal endometrial tissues （25 cases）, atypical hyperplasia of endometrial tis？sues （20 cases） and endometrial cancer tissues （40 cases）. Correlations between Drosha mRNA transcription and protein ex？pression with clinicopathological characteristics of EC were analyzed. Results The levels of Drosha mRNA and protein lev？els in EC were obviously lower than those in endometrial atypical hyperplasia and normal endometrium （P〈0.05）. But there is no significant difference of Drosha expression between endometrial atypical hyperplasia and normal endometrium tissues （P〉0.05）. The protein expression levels of Drosha were consistent with transcription of mRNA transcription levels. Drosha mRNA expression does not differ significantly with differentiation, histological type, myometrial invasion, lymphatic metasta？sis and FIGO stages of EC （P〉0.05）. Conclusion The expression levels of Drosha in EC tissues were down-regulated, therefore the reduction of Drosha may contributed to tumorigenesis of EC.