骨髓单个核细胞移植治疗2型糖尿病小鼠急性心肌梗死的研究

Bone Marrow Mononuclear Cells Transplant for the Treatment of Acute Myocardial Infarction in Mice with Type 2 Diabetes Mellitus

目的研究骨髓单个核细胞(BMMNCs)对T2DM小鼠心肌梗死的疗效.方法小鼠分为4组:正常小鼠注射盐水组(NS组),正常小鼠注射细胞组(NM组),糖尿病小鼠注射盐水组(TM组),糖尿病小鼠注射细胞组(TS组).取材后通过HE、Masson’s、v WF、Isolectin、WGA免疫染色观察疗效.结果正常组和糖尿病组细胞移植后VEGF表达水平相当,差异无统计学意义(P>0.05);细胞组梗死面积较盐水组小,胶原面积较盐水组的少,血管密度较盐水组大,心肌细胞肥大较盐水组轻,差异有统计学意义(P<0.05).结论骨髓单个核细胞对心肌损伤组织的修复、梗死边缘区细胞的肥大增生、血管新生方面有明显的改善作用.

Objective To study the curative effect of bone marrow mononuclear cells （BMMNCs） on acute myocardial infarction in mice with type 2 diabetes mellitus （T2DM） Methods Mice were divided into 4 groups： normal mice with saline injection, normal mice with cell injection, T2DM mice with saline injection, and T2DM mice with cell injection. The curative effect was observed through HE, Masson＇s, vWF, isolectin and WGA immunostaining after sampling. Results VEGF expression in T2DM group was similar in normal group after transplant, with no significant difference. The infarct and collagen size sizes in normal and T2DM groups after transplant were less than those in saline groups, blood vessel density was thicker than that in saline groups, cardiomyocyte hypertrophy was less severe than that in saline groups. The differences were statistical significance between cell transplant groups and saline groups （P 〈 0.05） . Conclusion The therapy of BMMNCs transplant has obvious improvement on the restoration of myocardial tissue damage, cell hypertrophy of the edge area of infarction and angiogenesis.