电针对大鼠脊髓损伤后损伤处远端少突胶质细胞的影响

Effect of Electro-acupuncture Treatment on Oligodendrocyte in the Remote Segment of Spinal Cord Injury Rats

目的探讨电针对大鼠脊髓损伤后损伤处少突胶质细胞的影响,为探索电针治疗脊髓损伤提供实验依据.方法将60只SD大鼠随机分为3组,即电针治疗组(EA)、脊髓损伤组(SCI)、对照组(SHAM),每组各20只大鼠.EA组接受SCI手术+EA治疗;SCI组接受SCI手术;SHAM组仅接受椎板切除术.在术后24h,术后7d、14d、21d、28d取脊髓损伤处组织行免疫组化染色,测定APC阳性细胞数的表达变化.结果同一时间点不同组之间,少突胶质细胞数目比较,电针组明显高于其他组;组之间的对比差异有统计学意义(P<0.05).电针组之间不同时间点对比,随着电针时间的增长,细胞数不断增长;不同时间点之间的差异有统计学意义(P<0.05).结论电针能促使大鼠脊髓损伤后损伤处远端少突胶质细胞的增殖与分化.

Objective The study was aimed to research the expression of oligodendrocyte in the remote segment of the spinal cord injury （SCI） rats model after the electro-acupuncture treatment, in order to provide an experimental basis for electro-acupuncture treatment of SCI. Methods 60 SD rats were randomly divided into the SCI group, the SHAM group and the electro-acupuncture （EA） group. The tissue materials of acute SCI model were obtained on the 7th, 14th, 21st and 28th day respectively after the injury. The expression of the oligodendrocyte in the injured spinal cord tissue in each group was observed by DAB staining. Results At the same time, the number of oligodendrocyte in EA group was significantly higher than that in other groups, and the difference had statistical significance （P 〈 0.05） . In EA group, the number of cells had increased gradually as treatment time growing, and there were significant differences between different time points （P 〈 0.05） Conclusion Electric-acupuncture could induce the proliferation and differentiation of oligodendrocyte and its precursor cells in the remote segment of SCI rats.