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利用多重聚合酶链反应和多色毛细管电泳分析布氏菌多位点可变数目串联重复序列 被引量：7

MLVA-16 loci panel on Brucellaspp.using multiplex PCR and multicolor capillary electrophoresis
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摘要 目的传统的布鲁氏菌多点可变数目串联重复序列分析方法涉及16个位点(MLVA-16),包括单重聚合酶链反应和琼脂糖凝胶电泳,一直以来都作为标准的基因分型方法,用于布病的病原监测和流行病学调查。然而,目前这种传统方法工作量较大,实验室间结果不宜比较;尤其是对大规模菌株进行基因分型或面临突发疫情时,急需一种高通量且快速的方法。方法用多重PCR和多色毛细管电泳方法建立一种可靠的,高通量的且自动化程度较高的MLVA-16改良分型系统,用82株菌进行测试。结果这种改良的MLVA-16分型系统具有很高的准确性,且具有快速和高通量的特点。结论改良的MLVA-16分型系统可用于基层布病实验室,为布病的病原监测提供高效的工具。 The Multi Locus Variable Number Tandem Repeat Analysis 16 loci panel （MLVA-16）, involving singleplex PCRs and agarose gel electrophoresis, is the standard genotyping method for Brucella spp. used also for the Brucella interna- tional online database. To find a rapid and through-put genotyping method, modified MLVA-16 can be used. We described an alternative, reliable, high-throughput MLVA-16 protocol using multiplex PCRs and multicolor capillary electrophoresis. Fur- thermore, 82 Brucella strains were tested. Results showed that the modified MLVA-16 has proved to be accurate, rapid and throughput. It＇s indicated that the modified MLVA-16 is highly discriminatory and epidemiological concordance and is easy for brucellosis surveillance in province-level laboratory.
作者 姜海 荣蓉 田国忠 赵娜 赵鸿雁 朴东日 赵赤鸿 崔步云
机构地区 中国疾病预防控制中心传染病预防控制所 中国疾病预防控制中心
出处 《中国人兽共患病学报》 CAS CSCD 北大核心 2015年第4期303-310,共8页 Chinese Journal of Zoonoses
基金 国家自然科学基金(No.81271900) 国家卫生计生委公益性行业科研专项(No.201302006) 重大专项(No.2012ZX10004215)~~
关键词 多重PCR 多色毛细管电泳 布鲁氏菌 基因分型 multiplex PCR multicolor capillary electrophoresis Brucella genotyping
分类号 R378.5 [医药卫生—病原生物学]
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参考文献18

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共引文献347

同被引文献60

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中国人兽共患病学报
2015年 第4期

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