TRAIL基因多态性与2型糖尿病、脂代谢紊乱的相关性

Correlation of TRAIL 1525G/A and 1595C/T polymorphisms with the risk of type 2 diabetes mellitus and lipid abnormalities

目的探讨肿瘤坏死因子凋亡相关诱导配体（TRAIL）基因1525G/A和1595C/T位点单核苷酸多态性（SNP）与汉族人2型糖尿病（T2DM）以及脂代谢紊乱的关系。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）结合DNA测序的方法检测TRAIL基因1525G/A位点和1595C/T位点多态性,其中对照组225例、2型糖尿病组201例和脂代谢紊乱组209例,分析各组间基因型及等位基因频率的差异,并分析空腹血糖（FPG）、餐后2 h血糖（2h PBG）、TG、TC、HDL-C、LDL-C等生化指标与各基因型之间的相关性。结果 TRAIL基因1525/1595位点多态性在T2DM组与对照组中基因型分布无明显差异（P〉0.05）。在脂代谢紊乱组与对照组中基因型分布差异显著（P=0.014）,脂代谢紊乱组中GG/CC和GA/CT基因型相对于AA/TT基因型发病风险增加,OR=1.842（95%CI=1.017~3.335）。在脂代谢紊乱组中,以AA/TT基因型为对照,GG/CC和GA/CT基因型中TC、HDL-C、LDL-C的血清学水平明显增高（P〈0.05）。结论 TRAIL基因多态性与T2DM无关,而与脂代谢紊乱有一定相关性,GG/CC和GA/CT基因型是脂代谢紊乱发生的危险因素。

The study performed to investigate the correlation of TRAIL gene 1525G/A and 1595C/T polymorphisms with the risk of type 2 diabetes（T2DM） mellitus and lipid abnormalities in Han Chinese. TRAIL gene 1525G/A and 1595C/T polymorphisms in case-control population including 201 cases with T2 DM, 209 cases with dyslipidemia and 225 control subjects were identified by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and DNA sequencing. The distribution of genotype and allele were analyzed in the groups, and the difference of FPG, 2h PBG, TC, TG, HDL-C, LDL-C and other parameters were analyzed among the people with different genotypes. The genotype and the allele frequencies of TRAIL gene 1525G/A and 1595C/T polymorphisms showed no significant difference between T2 DM and control groups（P〉 0.05）. While, there were significant differences in TRAIL genotype and allele frequencies between dyslipidemia and control groups（P=0.014）,and GG/CC genotype and GA/CT genotype increased the risk of lipid abnormality [OR=1.842（95%CI=1.017-3.335）].In the group of dyslipidemia, TC, HDL-C and LDL-C of GG/CC genotype and GA/CT genotype were higher than those of AA/TT genotype（P〈 0.05）. In conclusion, SNPs of TRAIL at 1525G/A and 1595C/T are associated with dyslipidemia, but not associated with T2 DM. GG/AA and GA/CT genotypes could increase the risk of lipid abnormality.