CCAAT/增强子结合蛋白β在系统性红斑狼疮患者外周血单个核细胞中的表达及意义

The expression and significance of CCAAT/enhancer binding proteins β in peripheral blood mononuclear cell of systemic lupus erythematosus

目的探讨系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中CCAAT/增强子结合蛋白(CCAAT/enhancer binding proteinsβ,C/EBPβ)的表达及其意义。方法从SLE患者血液中分离血浆和PBMC。通过定量PCR(quantitative PCR,q PCR)和Western blot方法检测SLE患者和健康对照PBMC中C/EBPβm RNA及其功能活性形式LAP(liver-enriched activating protein)蛋白表达水平。诱导THP-1细胞成为单核巨噬细胞,再分别用SLE患者血浆和健康对照血浆刺激24 h后,通过q PCR和Western blot方法检测C/EBPβm RNA和LAP蛋白表达水平。结果 SLE患者外周血PBMC中C/EBPβm RNA及LAP蛋白水平高于正常对照(P<0.05)。SLE患者外周血PBMC中C/EBPβm RNA表达水平及LAP蛋白灰度值与SLE疾病严重程度评分(SLEDAI评分)正相关(P<0.05,r>0)。与正常对照血浆相比,SLE患者血浆刺激THP-1细胞诱导成的单核巨噬细胞24 h后C/EBPβm RNA和LAP蛋白水平显著升高(P<0.05)。结论 SLE患者外周血PBMC中C/EBPβ表达显著升高,尤其是在单核巨噬细胞中,提示C/EBPβ表达升高参与SLE炎症发生的过程。

This study designed to explore the expression and significance of CCAAT/enhancer binding proteinsβ（C/EBPβ） in peripheral blood mononuclear cell（PBMC） of systemic lupus erythematosus（SLE）. Quantitative PCR（q PCR） and Western blot were used to observe C/EBPβ m RNA and LAP（liver-enriched activating protein）expression in peripheral blood mononuclear cell of SLE patient and healthy control. SLE patient plasma and healthy control plasma were used to stimulate the THP-1 derived monocyte-macrophage for 24 h. Then q PCR and Western blot were used to observe the m RNA and LAP protein expression of C/EBPβ. The difference was analyzed by the Mann-Whitney test; correlation analyses were conducted using Spearman＇s rank test. Results indicated that C/EBPβ m RNA and LAP protein expression in SLE patient PBMC were increased（P〈 0.05）. C/EBPβ m RNA expression level and LAP protein gray level positively correlated with SLEDAI（P 〈0.05,r〉 0）. SLE patient plasma stimulation upregulated C/EBPβ m RNA and LAP protein expression in THP-1 derived monocyte-macrophage（P〈 0.05）. In conclusion, the change of C/EBPβ expression, especially in monocyte-macrophage, contributes to the pathogenesis of SLE disease.