IL-1β基因启动子-31T/C多态性与子痫前期易感性的相关性研究

Relevant research between the interleukin- 1β gene promoter- 31( T / C )polymorphisms and the susceptibility of preeclampsia

目的探讨IL-1β基因启动子区-31T/C位点多态性与子痫前期发病的相关性。方法应用聚合酶链反应-限制性片段长度多态性（polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP）的方法检测232例子痫前期患者和447例正常对照组的IL-1β-31位点的基因型。人口流行病学资料和临床资料如年龄、怀孕次数、流产次数、收缩压等用t检验或非参数检验进行分析。2组间基因型及等位基因频率差异性比较采用χ2检验。结果 IL-1β-31位点基因型分布和等位基因频率在子痫前期组与对照组中的差异具有统计学意义（χ2=11.478,P=0.003）。C等位基因携带者发生子痫前期的危险性明显高于T等位基因携带者,CC基因型携带者患子痫前期的风险是非携带者的1.788倍（χ2=11.476,P=0.001,OR=1.788,95%CI=1.275~2.506）。结论IL-1β-31C/T位点多态性与子痫前期的遗传易感性相关,CC基因型携带者患子痫前期的风险较高。

The study designed to investigate the relationship between the IL-1β-31T/C polymorphisms and the susceptibility of preeclampsia（PE）. Polymorphisms at-31C/T of IL-1β were genotyped with the method of polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP） in 232 PE patients and 447 control subjects. Clinical data was analyzed using Student＇s test or Mann-Whitney test. The difference of genotype distribution and allele frequency between PE and control groups were analyzed by χ2test. Significant statistical differences were found between PE and control groups regarding genotype and allele frequencies of IL-1β-31 polymorphism. Subjects with C allele had an increased danger of developing preeclampsia compared with subjects with T allele. The risk of preeclampsia in the pregnant women with CC genotype was 1.788 times as compared with the pregnant women with the TT and CT genotypes（χ2=11.476, P =0.001, OR =1.788, 95% CI =1.275-2.506）. In codusion the polymorphism of the IL-1β-31T/C is associated with genetic susceptibility of preeclampsia in Chinese Han population. The pregnant women with CC genotype had a higher risk of preeclampsia.