应用Balb/c小鼠建立特应性皮炎的方法与评价

Establishment and evaluation of atopic dermatitis in Balb/c mice

目的探索应用变应原致敏Balb/c小鼠建立特应性皮炎(AD)的方法。方法不同剂量卵清蛋白(OVA)或2,4-二硝基氟苯(DNFB)经皮致敏Balb/c小鼠,经一定流程后观察皮肤症状并进行评分,H&E染色检测皮肤组织病理学变化,ELISA检测血清总Ig E含量,RT-PCR检测核因子T-bet和Gata3的m RNA表达。结果 OVA组(1、10、20、100 mg/ml)未见明显的皮肤炎症表现。DNFB组2种方案(0.5%+1%与0.5%)中,模型小鼠皮炎评分、抓搔次数、Ig E含量均显著升高(P<0.05),GATA3基因表达水平高于T-bet,呈Th2型免疫偏移状态,皮肤病理学检测观察到模型小鼠表皮增厚、棘层细胞增生、炎症细胞大量浸润等变化。结论本研究中,经皮给予DNFB能够成功诱导小鼠发生AD样改变,是进一步开展AD相关研究的基础。

To explore the method of establishing atopic dermatitis（AD） animal model using Balb/c mice,different doses of OVA or DNFB were administered transdermally to Balb/c mice. After counting the score, the histopathological changes of skin were measured by HE staining. Meanwhile, the level of Ig E in serum and the m RNA expressions of T-bet and Gata3 were analyzed by ELISA and RT-PCR, respectively. The results showed that there were no significant differences of cutaneous manifestations in four OVA groups（1, 10, 20, 100 mg/ml）.However, DNFB（0.5% ＋1% and 0.5%） could induce inflammation in skin significantly, characterized with higher level of Ig E and T-bet/Gata3 m RNA. The score and scratching number were also increased obviously（P〈 0.05）. The pathological detection indicated that epidermal thickness and prickle cell layer increased, accompanied with abundant inflammatory cell infiltration. All the result indicates that a mouse model of AD could be induced by DNFB percutaneously, which would provide a basis for AD-related research.