穿山龙醇提取物对高尿酸血症小鼠的治疗作用

Hypouricemic effect of ethanol extracts from Dioscoreae Nipponicae Rhizoma

目的：研究穿山龙醇提取物对高尿酸血症小鼠的降血尿酸作用。方法：分别用次黄嘌呤灌胃同时皮下注射氧嗪酸钾（模型A）和皮下注射尿酸（模型B）的方法建立两种小鼠高尿酸血症模型，并用穿山龙醇提取物干预两种模型，评估其降尿酸的效果。用高效液相色谱法测定血尿酸含量。结果：对于次黄嘌呤和氧嗪酸钾诱导的小鼠高尿酸血症模型，模型A组、空白对照组和穿山龙醇提取物组的血尿酸水平分别为（40．03±27．24）、（4．08±1．47）和（18．10±8．87） g／mL，穿山龙醇提取物组与模型组比较差异有统计学意义（ P＜0．05）；对于尿酸诱导的小鼠高尿酸血症模型，模型B组、空白对照组和穿山龙醇提取物组的血尿酸水平分别为（18．57±3．83）、（4．29±2．36）和（15．36±2．71）g／mL，穿山龙醇提取物组与模型组比较差异有统计学意义（ P＜0．05）。结论：对于上述两种高尿酸血症动物模型，穿山龙醇提取物组均有显著的降血尿酸作用。

Objective： To investigate the effect of ethanol extracts from Dioscoreae Nipponicae Rhizoma on hyperuricemic mice .Methods：The hyperuricemia was induced by gavage of hypoxanthine and subcutaneous injection of potassium oxonate （model A） or subcutaneous injection of uric acid （ model B ） in ICR male mice .The mice in ethanol extracts groups were administrated with Dioscoreae Nipponicae Rhizoma ethanol extracts 5.4 g/kg by gavage , the positive control groups were given with 10 mg/ml allopurinol or 5 mg/ml benzbromarone by gavage , respectively .The plasma uric acid levels were measured by using HPLC .Results： The plasma uric acid levels of model group, control group and ethanol extract group in model A mice were （ 40.03 ± 27.24）, （4.08 ±1.47） and （18.10 ±8.87） g/mL（compared with model group, P〈0.05 ） , respectively .The plasma uric acid levels of model group , control group and ethanol extract group in model B mice were （ 18.57 ±3.83 ） , （ 4.29 ±2.36 ） and （ 15.36 ±2.71 ） g/mL （ compared with model group , P 〈0.05 ） , respectively . Conclusion： The ethanol extracts from Dioscoreae Nipponicae Rhizoma have certain hypouricemic effect in hyperuricemic mice induced by hypoxanthine and potassium oxonate or by uric acid .