摘要
目的研究外阴阴道假丝酵母菌病(VVC)患者阴道内乳杆菌的种类以及各菌种在VVC患者阴道微环境中的分布特点。方法对象为2014年1月1日至10月1日就诊于北京医院妇科的女患者及在北京医院体检中心进行健康查体的女性,共154人,其中诊断VVC的患者62例,无自觉症状、无明确病史、微生态正常的女性92例。应用种特异性PCR技术分析14个常见阴道乳杆菌菌种的检出率。结果VVC女性阴道中各菌种分布为:惰性乳杆菌(6.5%)、卷曲乳杆菌(79.0%)、加氏乳杆菌(37.1%)、詹氏乳杆菌(74.2%)、嗜酸乳杆菌(16.1%)、短小乳杆菌(19.4%)、植物乳杆菌(1.6%)、约氏乳杆菌(51.6%)、发酵乳杆菌(8.1%)、唾液乳杆菌(9.7%)、罗伊氏乳杆菌(1.6%)和副干酪乳杆菌(8.1%)、德氏乳杆菌(3.2%);其中2%(1例)的患者分泌物中未检测出乳杆菌,35%(22例)含有乳杆菌2个菌种,63%(39例)含有3个以上菌种并存。育龄期健康女性阴道分泌物中惰性乳杆菌(82.6%)、卷曲乳杆菌(70.7%)、加氏乳杆菌(67.4%)、詹氏乳杆菌(40.2%)、嗜酸乳杆菌(39.1%)、短小乳杆菌(23.9%)、植物乳杆菌(5.4%)、约氏乳杆菌(3.3%)、发酵乳杆菌(2.2%)、唾液乳杆菌(2.2%)、鼠李糖乳杆菌(1.1%)、罗伊氏乳杆菌(1.1%)和副干酪乳杆菌(1.1%);3%(3例)的女性仅有1个乳杆菌菌种;15%(14例)女性阴道中同时有2个菌种并存,82%(75例)女性阴道内有3种或3种以上菌种并存。结论VVC患者阴道内乳杆菌的种类并未明显减少,惰性乳杆菌可能是阴道微环境改变的一个标志物。在育龄健康女性及VVC患者卷曲乳杆菌均为阴道内优势菌。
Objective To investigate the Lactobacillus species in the vaginas of vulvovaginal candidiasis and to assess the prevalence of each Laetobacillus species in vulvovaginal candidiasis. Methods 154 vaginal samples were analyzed, 92 of which were from fertile heahhy women, and 62 of which were from women with vulvovaginal candidiasis;and species-specific PCR showed the prevalence of each Lactobacillus species Species-specific PCR was used to investigate the prevalence of each Lactobacillus species in healthy Chinese women and the women with vulvovaginal candidiasis. Results In women with vulvovaginal eandidiasis: L. iners ( 6. 5% ), L cripatus ( 79.0% ), L. gasseri ( 37.1% ), L. jensenii ( 74. 2% ), L. aeidophilus(16.1%), Lhrevis (19.4%), L. plantarum (1.6%), L. johnsonii (51.6%), L fermentum(8.1% ), L. salivarius ( 9. 7% ), L renter ( 1.6% ), L. paracasei ( 8.1% ), L. delbrueckii (3.2%) ; More than two different Lactobacillus species coexisted in 98% of women with vulvovaginal candidiasis, and no anyone species existed in 2% of them;In fertile women: L iners(82. 6% ), L. eripatus (70. 7% ), L. gasseri(67. 4% ), L. jensenii(40. 2% ), L. acidophilus(39. 1% ), L. brevis(23.9% ), L. plantarum( 5. 4% ), L. rhamnosus ( 1.1% ), L. paracasei ( 1.1% ) , L. renter ( 1.1% ) i, L. johnsonii (3.3%), L. fermentum (2. 2% ), L. salivarius (2. 2% ) ; More than two different Lactobacillus species coexisted in 97% of fertile women, and only one species existed in 3% of fertile women. Conclusions Species of lactobacillus in women with vulvovaginal candidiasis did not significantly reduced compared with healthy women. Lactobacillus inert may be a marker of the change of vaginal microenvironment ; Lactobacillus erispatus is a dominant laetobacillus in the vaginal, of fertile healthy women, pregnant women and women with vulvovaginal candidiasis.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2015年第13期1012-1016,共5页
National Medical Journal of China