海藻糖对过氧化氢诱导M14黑素瘤细胞氧化损伤的保护作用

Protection of trehalose against damage of hydrogen peroxide to melanoma cell lines M14

目的:确定海藻糖对过氧化氢诱导的M14黑素瘤细胞损伤的保护作用。方法:不同浓度过氧化氢处理后的M14黑素瘤细胞加入0.5μg/mL、1μg/mL、10μg/mL海藻糖,采用MTT法检测各组细胞存活率,嘌呤氧化酶法检测处理前后超氧化物歧化酶(SOD)的活性。结果:浓度为1μg/mL的海藻糖抗氧化作用明显,可提高低浓度过氧化氢(小于400μmol/L)损伤后的M14黑素瘤细胞存活率约33%,可提高SOD活力约20%。当过氧化氢浓度大于400μmol/L时,各浓度海藻糖对提高细胞存活率无明显作用。结论:1μg/mL海藻糖对低浓度(小于400μmol/L)过氧化氢诱导的M14黑素瘤细胞损伤具有保护作用。

Objective：To determine the protective effect of trehalose against the damage of hydrogen peroxide to the melanoma cell lines M14. Methods：Melanoma cell lines M14 induced by hydrogen peroxide were recultured by different concentrations of trehalose （0.5 μg/mL, 1 μg/mL, 10 μg/mL）. The cell survival rate was detected by MTT. The activity of SOD was detected by xanthine oxidase technique. Results：The an-tioxidation of 1μg/mL trehalose was obvious. 1μg/mL trehalose improved the survival rate and the activity of SOD of M14 cells induced by low-concentration （〈400μmol/L） hydrogen peroxide by 33% and 20% respec-tively. The survival rate of M14 cells induced by high-concentration （〉400 μmol/L） hydrogen peroxide was not increased after re-cultured in different concentrations of trehalose. Conclusion：1μg/mL trehalose is pro-tective against the damage of low-concentration （〈400 μmol/L ） hydrogen peroxide to the melanoma cell lines M14.