BDNF对食管癌细胞株ECa9706体外侵袭能力的影响

Effect of brain-derived neurotrophic factor on in vitro metastasis of esophageal carcinoma cell line ECa9706

目的:本研究观察脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)对食管癌细胞株ECa9706体外增殖和迁移能力的影响以及机制.方法:采用MTT和Transwell方法分别观察BDNF以及酪氨酸激酶受体B(tyrosine kinase receptor B,Trk B)抑制剂K252a对ECa9706细胞增殖和迁移能力的影响,采用实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,q PCR)和Western blot方法分别检测ECa9706细胞中Trk B和磷脂酶C(phospholipase C,PLC)-γ1的m RNA和蛋白表达水平.结果:BDNF可显著促进ECa9706细胞增殖和迁移能力,该作用可被K252a拮抗.与对照组相比,BDNF可诱导Trk B的m RNA和蛋白表达显著增加,并提高PLC-γ1的蛋白表达水平.结论:BDNF/Trk B/PLC-γ1在食管癌细胞迁移浸润中具有重要作用.

AIM： To explore the effect of brain-derived neurotrophic factor（BDNF） on the proliferation and migration of ECa9706 cell line in vitro and the underlying mechanism. METHODS： Cell proliferation and migration were examined by MTT assay and transwell assay, respectively. The m RNA and protein expression levels of tyrosine kinase receptorB（Trk B） and phospholipase C（PLC）-γ1 were detected by real-time quantitative polymerase chain reaction（q PCR） and Western blot. RESULTS： BDNF enhanced the proliferation and migration of ECa9706 cells significantly in a dose-dependent manner, and this effect was blocked by K252a（a Trk B antagonist）. Compared with control cells, BDNF increased the m RNA and protein expression of Trk B, and the protein expression of PLC-γ1.