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RNA-Seq技术在瘤胃微生物研究中的应用进展 被引量：3

RNA-Seq Technology and Its Application in Rumen Microbes
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摘要 反刍动物瘤胃内多种多样的微生物组成一个复杂的微生态系统,它们在瘤胃内物质消化过程中发挥重要作用。RNA-Seq技术的出现为反刍动物瘤胃微生物的研究提供了前所未有的机遇。本文主要从新一代高通量测序技术出发,阐述RNA-Seq技术在瘤胃微生物代谢酶特性、瘤胃微生物的多样性、瘤胃微生物新功能基因方面的应用,以期为瘤胃微生物的研究提供参考。 Varied microbes species in the rumen compose a complex microecosystem, which play an impor-tant role in the process of rumen digestion. The appearance of RNA-Seq technology has provided huge op-portunities for studying on rumen microbes. This review mainly described how to study on enzyme metabolic characteristics of rumen microbes,the diversity of rumen microorganism,new functional genes of rumen mi-crobes using RNA-Seq technology with next-generation sequencing technology.
作者 廖奇 刘旭川 李清 樊月圆 张春勇 杨舒黎 毛华明 冷静
机构地区 云南省动物营养与饲料科学重点实验室 云南农业大学动物科学技术学院
出处 《动物营养学报》 CAS CSCD 北大核心 2015年第4期1061-1067,共7页 CHINESE JOURNAL OF ANIMAL NUTRITION
基金 云南省自然科学基金重点项目(2014FA033) 云南省自然科学基金项目(2010CD059) 国家自然科学基金项目(31060314) 云南省中青年学术和技术带头人后备人才项目(2011HB025)
关键词 RNA-Seq技术 瘤胃微生物 多样性 新功能基因 RNA-Seq technology rumen microbes diversity new functional genes
分类号 Q51 [生物学—生物化学]
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参考文献45

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动物营养学报
2015年 第4期

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