正骨消瘀散定性定量方法研究

Qualitative and quantitative method for Zhenggu Xiaoyu powder

目的建立正骨消瘀散的定性定量方法。方法采用薄层色谱法对制剂中当归、防风、大黄、侧柏叶4味药材进行定性鉴别;采用C18柱,流动相甲醇-1%醋酸（25：75）,检测波长316 nm测定样品中阿魏酸的含量;采用C18柱,流动相乙腈-水（32：68）,检测波长290 nm测定样品中桂皮醛的含量。结果薄层鉴别的色谱斑点清晰,阴性对照无干扰;阿魏酸在0.024~0.144μg与峰面积呈良好的线性关系（r=0.999 8）,平均加样回收率为99.6%,RSD为1.8%;桂皮醛在0.02~0.12μg与峰面积呈良好的线性关系（r=0.999 8）,平均加样回收率为98.6%,RSD为1.5%,精密度和重复性良好。结论该方法操作简便,重复性好,专属性强,可以用于正骨消瘀散的质量控制。

Objective To establish a qualitative and quantitative method for Zhenggu Xiaoyu powder. MethodsChinses Angelica, Saposhnikoviadivaricata, Rheum officinale and Cacumenbiotae were identified by TLC, and the content of ferulic acid in the powder was determined by HPLC with C18 column. The mobile phase consisted of methanol-1% acetic acid（25 ：75）. The detection wavelength was 316 nm; The content of cinnamaldehyde in the powder was determined by HPLC with C18 column, the mobile phase consisted of acetonitrile-water（32 ：68）, and the detection wavelength was 290 nm. Results Chinses Angelica, Saposhnikoviadivaricata, Rheum officinale and Cacumenbiotae were identified by TLC. The calibration curve of ferulic acid was in a good linearity at 0.024-0.144 μg（r = 0.999 8）. The average recovery was 99.6% with RSD of 1.8%（n = 6）. The calibration curve of cinnamaldehyde was in a good linearity at 0.02- 0.12 μg（r = 0.999 8）. The average recovery was 98.6% with RSDof 1.5%（n = 6）. Conclusion The method is simple, accurate, and can be used for the quality control of Zhenggu Xiaoyu powder.