摘要
目的建立鼻咽清毒剂中4种活性成分木犀草苷、蒙花苷、噻嗪二酮苷和3,5-O-二咖啡酰基奎宁酸的含量测定方法。方法依利特C18色谱柱(4.6 mm×250 mm,5μm);流动相为甲醇-0.1%磷酸溶液,梯度洗脱,流速0.9 m L·min-1;检测波长334 nm(木犀草苷、蒙花苷);254 nm(噻嗪二酮苷、3,5-O-二咖啡酰基奎宁酸);柱温30℃。结果在该色谱条件下,木犀草苷、蒙花苷、噻嗪二酮苷和3,5-O-二咖啡酰基奎宁酸分离良好,质量浓度分别在9.84~196.80μg·m L-1(r=0.999 8)、11.92~238.40μg·m L-1(r=0.999 6)、6.85~137.00μg·m L-1(r=0.999 9)、5.60~112.00μg·m L-1(r=0.999 4)时与其峰面积呈良好的线性关系,加样回收率依次为96.9%、99.0%、97.8%、96.9%,RSD依次为1.4%、1.4%、1.6%、0.71%(n=6)。结论所建立的方法简便、准确、重复性好,可用于鼻咽清毒剂的质量控制。
Objective To determine the content of 4 ingredients in Biyan Qingdu granule was developed by HPLC method. Methods HPLC separation was performed on Hypersil C18 column(4.6 mm×250 mm, 5 μm) with a gradient elution method, 0.1% phosphoric acid solution was used as the mobile phase at 0.9 m L·min- 1. The column temperature was 30 ℃. The wavelenth was 334 nm for luteolin-7-O-glucoside, buddleoside and 254 nm for xanthiside,(-)-3, 5-dicaffeoyl quinic acid. Results Luteolin-7-O-glucoside, buddleoside, xanthiside and(-)-3, 5-dicaffeoyl quinic acid were baseline separated and have a good linearity at 9.84- 196.80 μg·m L- 1(r =0.999 8), 11.92- 238.40 μg·m L- 1(r = 0.999 6), 6.85- 137.00 μg·m L- 1(r = 0.999 9), and 5.60- 112.00 μg·m L- 1(r = 0.999 4), respectively. The average recoveries and RSDs were 96.9%(1.4%), 99.0%(1.4%), 97.8%(1.6%) and 96.9%(0.71%), respectively. Conclusion The method is simple, accurate, and shows good precision and reproducibility, which can be used for the quality control of Biyan Qingdu granules.
出处
《中南药学》
CAS
2015年第3期303-306,共4页
Central South Pharmacy
基金
广西壮族自治区教育厅高等教育教学改革工程项目-药品检验技术课程整体教学设计研究与实践[(No.桂教高教(2014)15号)]
关键词
鼻咽清毒剂
木犀草苷
蒙花苷
噻嗪二酮苷
3
5-O-二咖啡酰基奎宁酸
野菊花
苍耳子
Biyan Qingdu granule
luteolin-7-O-glucoside
buddleoside
xanthiside
(-)-3
5-dicaffeoyl quinic acid
chrysanthemi indici flos
xanthii fructus