摘要
[目的]了解我国主要生猪养殖地区猪群中猪伪狂犬病毒感染情况。[方法]分别采用PCR和gEELISA检测方法,对2012-2013年采集/送检的1127份发病猪群组织样品、14801份猪血清样品进行了病原学、血清学检测。[结果]检出PRV野毒感染病原学样品98份,总体检出率8.69%,猪场的PCR检出率为22.49%;共检测14801份血清样品,野毒感染抗体阳性样品2388份,总体阳性率为16.13%,场的阳性率为44.02%。对检测数据进行分类统计,发现华东、华中等生猪主产区猪群中均存在不同程度的PRV野毒感染,感染率呈逐年上升趋势;分析不同日龄猪群的野毒感染抗体检测数据,发现种猪的感染率较高,其次是哺乳仔猪、育肥猪。[结论]猪伪狂犬病在我国主要生猪养殖地区仍不同程度存在,提示应进一步提升猪伪狂犬病的免疫预防水平,定期进行病原学、血清学检测,做好综合防治工作。
[Objective] The aim of the study is to understand the porcine pseudorabies virus infection in the major pigproducing areas in China. [Method] PCR and gE-ELISA were performed to test 1127 tissue samples and serum samples respectively in 2012 to 2013. [Result] 98 PRV positive tissue samples were detected from 1127 tissue samples with an overall detection rate of 8.69%,and an overall farm positive rate of 22.49%;on the other hand,2388 gE-ELISA positive serum samples（the wild PRV infected samples） were detected from 14801 serum samples with an overall detection rate of 16.13%,and an overall farm positive rate of 44.02%. Classification of all the test data showed that wild PRV infection existed in many pig-farms in east and central China with an upward tendency infection rate. Analysis of the gEELISA data of serum samples collected from different pig ages revealed that the wild PRV infection rate in boar and sow pigs was high,followed by piglets and growing pigs.[Conclusion]The results showed that the porcine pseudorabies virus still existed in pig-farms of major pig-producing areas in China,and it is recommended to enhance the pseudorabies immunization,prevention and control level,to conduct the etiological and serological surveillance and to carry out comprehensive prevention and control measures for the disease.
出处
《中国动物检疫》
CAS
2015年第4期1-5,共5页
China Animal Health Inspection
基金
青岛市民生计划项目13-1-3-83-nsh
