银杏叶提取物对实验性自身免疫性脑脊髓炎大鼠的作用研究

The effects of extract of Ginkgo biloba on the experimental autoimmune encephalomyelitis of rat

目的:建立雌性Wistar大鼠实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)模型,观察银杏叶提取物EGb761腹腔注射对EAE大鼠的可能疗效及神经保护作用和机制。方法:建立Wistar大鼠EAE模型,腹腔注射EGb761(70 mg/kg),连续7 d,于免疫后第14 d和第31 d取颈膨大,分别进行HE、LFB和改良BLS染色,观察各组大鼠炎细胞浸润、髓鞘脱失和轴突缺失情况。结果:(1)EGb761组与EAE组相比临床评分明显减低(P<0.05);(2)HE染色显示:EGb761组炎症浸润与EAE组相比明显减低(P<0.01);(3)LFB染色显示:EGb761脱髓鞘评分与EAE组相比明显降低(P<0.05);(4)改良Bielschowsky染色显示:EGb761组高峰期轴突平均缺失面积与EAE组相比明显下降(P<0.01)。结论:EGb761通过减轻炎细胞浸润、促进髓鞘再生和防止轴突缺失,从而对EAE大鼠临床症状的改善起到一定作用。

Objective： By the establishment of EAE（ experimental autoimmune encephalomyelitis,EAE） model on female Wistar rats,to evaluate the effects of Ginkgo biloba extra（ EGb761） on the EAE and observe the mechanism of nerve protective effect. Methods： Curative and neural protective EAE model on Wistar rats was made,and then intraperitoneal injection of EGb761（ 70 mg / kg） for continuous 7 days was performed. At 14 d and 31 d post immunization the cervical enlargement was remaved,then the inflammatory infiltration,demyelination,and axonal loss was observed by means of hematoxylin-eosin staining（ HE）,luxol fast blue（ LFB） and modified Bielschowsky silver staining（ BLS）. Results：（ 1） The clinical score of group EGb761,compared with that of the EAE group,was significantly decreased（ P 〈0. 05）.（ 2） HE staining showed that the inflammatory infiltration of the group EGb761,compared with that of EAE group,was significantly decreased（ P 〈 0. 01）.（ 3） LFB staining showed that group EGb761,compared with the EAE group,the demyelinating score was significantly decreased（ P 〈 0. 05）.（ 4） Modified Bielschowsky staining showed that group EGb761 significantly reduced with respect to the average axonal deletion areas during the peak period compared with that of EAE group（ P 〈 0. 01）. Conclusion： The EGb761 plays a certain role in improving the clinical symptoms of EAE in rat through reducing the inflammatory infiltration,promoting remyelination and preventing axonal loss.