胶质纤维酸性蛋白及生长相关蛋白-43在低声压次声治疗颅脑外伤大鼠过程中的变化

The dynamic expression of glial fibzillary acidic protein and growth associated protein- 43 in rats with traumatic brain injury after being treated by infrasound

目的:探讨低声压级次声对大鼠颅脑外伤后胶质纤维蛋白(GFAP)及生长相关蛋白-43(GAP-43)表达的影响。方法:采用Feeney法制作颅脑外伤模型。将30只SD大鼠随机分为假手术组、模型组、次声60min组、次声90min组和次声120min组共5组,其中次声3组分别予次声干预60min、90min和120min,连续7天;模型组干预过程中除不开机外,其余过程同次声组。假手术组只打开颅窗,不损伤脑组织,不进行次声干预。7天后处死前行改良神经功能缺损评分(m NSS),然后断头取脑、免疫组化观察损伤脑组织周围GFAP及GAP-43表达变化。结果:次声3组与模型组的m NSS评分均存在显著差异(P<0.05),次声3组间的m NSS评分无显著差异(P>0.05)。免疫组化结果:15组间的GFAP表达存在显著性差异(P<0.05),3个次声组与模型组比较均存在显著差异(P<0.05),3个次声组间GFAP表达无明显差异(P>0.05);25组间的GAP-43表达存在显著性差异(P<0.05),3个次声组与模型组比较均存在显著差异(P<0.05),3个次声组间GAP-43表达无明显差异(P>0.05)。结论:低声压级次声能提高大鼠脑外伤后脑组织GFAP及GAP-43的表达,改善脑外伤大鼠神经功能。

Objective： To explore the effect of infrasound on expressions of glial fibrillary acidic protein （GFAP） and growth associated protein-43（GAP-43） after traumatic brain injury（TBI） in rats. Method： The model was constructed using the modified Feeney＇s free falling weight TBI model. Thirty male adult SD rats were randomly divided into sham group （n=6）, model group （n--6）, 60min infrasound group （n= 6）, 90rain infrasound group （n=6） and 120min infrasound group （n=6）. The infrasound group were individually treated with infrasound for 30min, 90min and 120min after operation for 7 days. The model group was treated in the same way except turning off the power. The sham group was not hurted, no intervention. Rats were evaluated with the modified neurological severity score （mNSS） after 7 days, and the brain tissue slices were immunohistochemically stained to observe the expressions of GFAP and GAP-43 around the injury areas. Result： The mNSS score were different between infrasound groups and model group （P〈 0.05）. There was no difference among the infrasound groups （P〉 0.05）. Immunohistochemical results： ①The integral optical density （IOD） of GFAP was significantly different among the 5 groups （P〈 0.05）; there were significant differences among the infrasound groups and the model group （P 〈 0.05）; there was no significant difference within the in-frasound groups （P〉 0.05）. ② The IOD of GAP-43 were significantly different among the 5 groups （P〈 0.05）. There were significantly differences among the infrasound groups and the model group （P〈 0.05）. There was no significant difference within the infrasound groups （P 〉 0.05）. Conclusion： Infrasound can increase the expressions of GFAP and GAP-43 and restore the brain injury and im- prove the neurological function after TBI in rats.