后房型有晶状体眼人工晶状体植入对兔眼晶状体上皮细胞及房水流动的影响

The influence of posterior chamber phakic intraocular lens on lens epithelium and aqueous flow of the implanted rabbit eyes

目的通过动物实验了解后房型有晶状体眼人工晶状体(PC-PIOL)植入对眼内邻近组织及房水流动的影响。设计实验研究。研究对象20只新西兰标准实验兔(20眼)。方法将20只实验兔随机分为2组,每组10只,均以右眼为实验眼。实验组在植入PC-PIOL后给予局部药物治疗,待裂隙灯显微镜检查房水闪光呈现阴性后,经UBM引导将示踪剂分别注入两组动物右眼后房,手术显微镜下观察示踪剂在前房出现的例数及出现时间。对照组不植入人工晶状体,在实验组炎症消失后,与实验组同时进行以上相关检查。此后分别取两组实验动物的右眼晶状体前囊膜进行透射电镜检查。主要指标透射电镜检查结果,示踪剂到达前房的例数及时间。结果两组实验动物晶状体上皮细胞在透射电镜下未见明显形态差异。注射示踪剂后,实验组4例(4眼)和对照组5例(5眼)在前房观察到示踪剂(χ2=0.639,P=0.412)。实验组的示踪剂经注射后在前房出现的时间为(3.07±0.03)秒,对照组为(2.42±0.08)秒(t=1.413,P=0.081)。结论 PC-PIOL眼内植入在术后短时间内未对晶状体上皮细胞产生明显影响;对房水从后房向前房流通速度无明显影响。

Objective To observe the influence of posterior chamber phakic intraocular lens （PC-PIOL） on the eye tissue and aqueous flow of the implanted eyes. Design Experimental study. Participant 20 New Zealand standard experiment rabbits （20 eyes）. Methods 20 experiment rabbits were randomly divided into 2 groups, 10 rabbits each group, and the right eye in each group was observed PC-PIOL implanted only in eyes of experimental group. After inflammation caused by surgery was well controlled, tracer element was injected into posterior chamber of the experimental eye, and the time of its appearance in anterior chamber was recorded for the two groups. Then the anterior capsular membrane of right eyes in two groups was taken for examination with electron microscope. Main Outcome Measures Proportion of eyes with tracer element appearing in anterior chamber corresponding time cost for tracer appearing, and the morphology of capsular cell. Results The tracer element appeared in anterior chamber in 4 eyes in experiment group, and 5 eyes in control group. The difference was not significant （χ^2=0.639, P=0.412）. The time of tracer appearance was （3.07±0.03） seconds in experiment group, and （2.42±0.08） seconds in control group （t=1.413, P=-0.081）. The cell morphology shows no unusual appearance between both groups. Conclusion The implantation of PC-PIOL did not induce the significant impact on capsular cell morphology short time after surgery, and did not obstruct acquous flow from posterior chamber to anterior chamber.