IL-37抑制脂多糖诱导的小鼠树突状细胞活化

Interleukin-37 inhibits the activation of mouse dendritic cells induced by lipopolysaccharides

目的探讨白细胞介素37(IL-37)对细菌脂多糖(LPS)诱导的小鼠树突状细胞(DC)活化的调节作用。方法应用GM-CSF和IL-4诱导小鼠骨髓细胞向DC分化,抗CD11c磁珠分选DC。IL-37预处理DC后,进行LPS刺激。流式细胞术检测DC表面共刺激分子(CD80、CD86)表达水平,实时荧光定量PCR检测肿瘤坏死因子α(TNF-α)、IL-6和IL-1αmRNA表达水平,流式细胞微球芯片试剂盒(CBA试剂盒)检测细胞培养上清中IL-1α、IL-6、TNF-α等因子的浓度。结果 DC诱导成功,磁珠分选能够获得高纯度的DC(>90%)。IL-37降低LPS诱导的DC表面共刺激分子CD80、CD86的表达,并抑制DC合成IL-1α、IL-6、TNF-α。结论 IL-37可以通过降低共刺激分子和炎症因子的表达抑制LPS刺激的DC活化。

Objective To investigate the role of interleukin-37（IL-37） in regulating the activation of mouse dendritic cells（DCs） induced by lipopolysaccharides（LPS）.Methods The mouse bone marrow cells was induced to differentiate into DCs with GM-CSF and IL-4.DCs were purified with anti-CD11 c immunomagnetic beads.After pretreated with IL-37,DCs were stimulated by LPS.Then the levels of co-stimulatory molecules CD80 and CD86 on DCs were detected by flow cytometry.The mRNA levels of inflammation cytokines tumor necrosis factor α（TNF-α）,IL-6 and IL-1α were determined by real-time quantitative PCR.The protein levels of inflammatory cytokines IL-1α,IL-6,TNF-α were measured by cytometric beads array（CBA） kit.Results We induced successfully DCs and obtained a high purity of DCs（ 〉90%） with anti-CD11 c immunomagnetic beads.After stimulated by LPS,the levels of co-stimulating molecules CD80,CD86 on IL-37-treated DCs were reduced,and the expressions of the cytokines TNF-α,IL-6 and IL-1α were also down-regulated at both mRNA and protein levels.Conclusion IL-37 plays a critical role in inhibiting LPS-induced DCs activation via suppressing production of co-stimulatory molecules CD80,CD86 and pro-inflammatory cytokines.