荷瘤小鼠卵巢癌细胞miRNA和髓源性抑制细胞的检测及相关性分析

Detection and correlation analysis of miRNAs and myeloid-derived suppressor cells in ovarian cancer-bearing mice

目的检测卵巢癌荷瘤小鼠癌组织miR-21-5p、miR-155-5p、miR-218-5p、miR-222-3p、miR-494-3p的水平和外周血和脾脏中髓源性抑制细胞(MDSC)的数量,并分析它们的相关性。方法 12例卵巢癌荷瘤小鼠的癌组织和癌旁组织,实时荧光定量PCR检测癌组织和癌旁组织中miR-21a-5p、miR-155a-5p、miR-218-5p、miR-222-3p、miR-494-3p的水平,流式细胞术检测上述荷瘤小鼠中外周血和脾脏中MDSC的表达。采用Spearman法分析MDSC和microRNA的相关性。结果与正常小鼠相比,荷瘤小鼠外周血和脾脏中的MDSC明显增加。miR-21a-5p、miR-218-5p和miR-222-3p在癌组织中含量高于癌旁组织,相反miR-155a-5p和miR-494-3p在癌组织中的表达低于癌旁组织。miR-222-3p与MDSC的数量无相关性,miR-494-3p的水平与MDSC的数量负相关,miR-21a-5p、miR-155a-5p、miR-218-5p在癌组织与癌旁组织的表达差值与MDSC的数量呈正相关。结论卵巢癌荷瘤小鼠癌组织中miR-21a-5p、miR-155a-5p、miR-218-5p、miR-494-3p水平和外周血及脾脏中MDSC的数量有一定的相关性。

Objective To detect the levels of miR-21a-5p,miR-155-5p,miR-218-5p,miR-222-3p,miR-494-3p in ovarian cancer tissues and the number of myeloid-derived suppressor cells（MDSCs） in peripheral blood and spleen in ovarian cancerbearing mice,and explore their clinical significance and correlations.Methods The mRNA expressions of miR-21a-5p,miR-155a-5p,miR-218-5p,miR-222-3p,miR-494-3p were detected by real-time quantitative PCR（qRT-PCR） in tumor tissues and tumor-adjacent normal tissues from 12 ovarian cancer-bearing mice.The frequency of MDSCs in the peripheral blood and spleen from the 12 tumor-bearing mice was measured by flow cytometry.Spearman correlation analysis was used to find out the correlations between MDSCs and miRNAs.Results Compared with the normal mice,the number of MDSCs in the peripheral blood and spleen of the tumor-bearing mice significantly increased.The levels of miR-21a-5p,miR-218-5p and miR-222-3p in tumor tissues were significantly higher than those in tumor-adjacent normal tissues; conversely,miR-155a-5p and miR-494-3p levels in the former were significantly lower than those in the latter.There was no correlation between miR-222-3p and MDSCs,but miR-494-3p had a negative correlation with MDSCs.The expression differences of miR-21a-5p,miR-155a-5p,miR-218-5p between tumor tissues and tumor-adjacent normal tissues had positive correlations with the number of MDSCs.Conclusion The levels of miR-21a-5p,miR-155a-5p,miR-218-5p,miR-494-3p in tumor tissues had correlations with the number of MDSCs in the peripheral blood and spleen of ovarian cancer-bearing mice.