血管内皮生长因子基因多态性与2型糖尿病视网膜病变相关性的Meta分析

A Meta-analysis of correlation between the polymorphism of vascular endothelial growth factor(VEGF) and diabetic retinopathy in type Ⅱ diabetes

目的利用Meta分析探讨血管内皮生长因子1(Vascular endothelial growth factor,VEGF)基因多态性与2型糖尿病视网膜病变的相关性。方法电子检索CNKI、万方及Pub Med数据库,收集有关VEGF936C/T及VEGF 460C/T基因多态性与2型糖尿病视网膜病变的病例对照研究。检索时间为建库至2013年9月。采用Rev Man5.0软件进行Meta分析。结果共纳入9个研究,其中5个研究的是VEGF 936C/T多态性与2型糖尿病视网膜病变的关系,4个研究的是VEGF 460C/T与2型糖尿病视网膜病变的关系。Meta分析结果显示:具有VEGF 460TT基因型[OR=0.31,95%CI(0.19,0.50),P<0.01],CT基因型[OR=0.67,95%CI(0.46,0.98),P=0.04]及TT+CT基因型[OR=0.43,95%CI(0.27,0.69),P=0.0005]的人群发生2型糖尿病视网膜病变的风险明显低于具有基因型CC的人群,另外VEGF 460T等位基因携带者2型糖尿病视网膜病变发病风险低于等位基因C携带者[OR=0.67,95%CI(0.55,0.81),P<0.01]。而VEGF 936C/T多态性在病例组和对照组分析差异无统计学意义(P>0.05)。结论 VEGF 460C/T基因多态性与2型糖尿病视网膜病变发病相关,VEGF 460C/T基因可能是2型糖尿病视网膜病变的遗传保护因素。

OBJECTIVE To investigate the association between the polymorphism of Vascular endothelial growth factor（VEGF） gene and susceptibility of diabetic retinopathy（DR） among patients with type 2 diabetes mellitus（T2DM）via meta-analysis. METHODS An electronically search based on CNKI,Wanfang database and Pub Med from their establishment to January 2013 was performed to collect case-control studies on VEGF 936C/T and VEGF 460C/T polymorphism and DR in T2 DM. Rev Man5.0 was used for this study. RESULTS Nine case-control studies were identified（five eligible studies were analyzed for VEGF 936C/T polymorphism and DR in T2 DM and fore were analyzed for VEGF 460C/T polymorphism and DR in T2DM） on association of VEGF polymorphism and DR in T2 DM. The results of meta-analysis showed that the population having the genotype VEGF 460 TT [OR=0.31,95% CI（0.19,0.50）,P〈0.00001],genotype CT [OR =0.67,95% CI（0.46,0.98）and TT ＋CT [OR =0.43,95% CI（0.27,0.69）,P=0.0005] presented an decreased DR risk compared to that with the genotypes CC. Moreover the al lele T showed significant association with decreased DR risk compared to the allele C [OR=0.67,95%CI（0.55,0.81）,P 〈0.00001]. While,no significant association was observed on the VEGF 936C/T polymorphism and DR in T2DM（P〉0.05）. CONCLUSIONS VEGF 460C/T polymorphisms was associated with DR in T2 DM and VEGF 460C/T variation which might be a genetic protective factor for DR in T2 DM.