壮骨康齿胶囊对大鼠雌二醇含量及牙周组织的影响

Effect of Zhuanggukangchi capsule, a preparation of traditional chinese medicine, on estrogen level and periodontal tissue in rats

目的观察壮骨康齿胶囊对大鼠雌二醇含量及牙周组织发育的影响。方法选用40只雌性大鼠随机分成两组 ,每组20只 ,用药组每日灌胃壮骨康齿胶囊配制的药液1ml(含药量30mg) ,对照组每日灌胃蒸馏水1ml,连续30天后 ,取血测定两组大鼠雌二醇含量 ,解剖子宫卵巢称重 ,计算子宫重量指数 ;选择32只雌性大鼠分两组 ,每组16只 ,喂药方法同前 ,分别于第1、4、8、12周处死大鼠 ,取下颌前牙的牙周组织作组织学切片 ,光镜观察。结果实验用药组雌二醇含量、子宫卵巢重量 ,子宫重量指数均明显高于对照组(P<0.01);用药组牙龈上皮、牙周膜明显增厚 ,肌纤维明显增粗。结论壮骨康齿胶囊可以明显提高大鼠雌二醇含量 。

Aim To investigate the effect of Zhanggukangchi capsule on estrogen level and the development of periodontal tissue in rats. Methods Forty female rats were divided into experimental and control groups in the former. Every rat was perfused with 1 ml Zhuanggukangchi fluid per day in the latter,and rats were perfused with distilled water, and 30 days later, the estradial content was detected, and the weight of rat body and their utero_ovarian were measured; In addition, another 32 female rats were also divided into experimental and control groups and fed as discribed aboue. Every 4 rats from each group were killed in 1,4,8 and 12 weeks respectively. The histological characteristics of periodontal tissue materials were observed with light microscope. Results The estradial content, weight of utero_ovariam, and index of uters/arium in experimental group were higher significantly than those in the control group (P<0.01) and gingival epthelial hyperplasia, pericementum and periodontal fibre were thickened. Conclusion It is revealed that Zhuanggukangchi capsule may increase extrogen level distinctly and promote the development of periodontal tissue.