高效液相色谱法分析吡咯喹啉醌

Determination of Pyrroloquinoline Quinine by High Performance Liquid Chromatography

目的：建立一种通过高效液相色谱（HPLC）定量测定吡咯喹啉醌（PQQ）的分析方法。方法：将PQQ标准品及发酵制备的PQQ结晶粉末溶于10 mmol/L NaOH溶液，利用高效液相色谱仪进行测定。采用Waters XBridge C18（4.6 mmx150 mm，5μm）作为分离柱，用甲醇-水（用三氟乙酸调节pH值为1.0）梯度洗脱，流速为1.0 mL/min，于室温下检测PQQ，检测波长为365.8 nm。结果：检测得PQQ标准品的保留时间约为7.8 min，在0.03125-1 mg/mL范围内线性关系良好，相关系数（r2）在0.9999以上，平均回收率为98.6%。结论：高效液相色谱法分析PQQ的灵敏度和准确度高，是一种可靠的PQQ定量分析方法。

Objective： To establish a quantitative analysis method for pyrroloquinoline quinone（PQQ） by high performance liquid chromatography（HPLC）. Methods： The PQQ standard and fermentation sample were dissolved with 10 mmol/L NaOH solution and analyzed by HPLC method. PQQ was seperated on a Waters XBridge C18 column（4.6 mmx150 mm,5 μm） with a gradient mobile phase（methanol and water, pH was adjusted to 1.0 by trifluoroacetic acid） at 1.0 mL/min flow rate. The detective wavelength was 365.8 nm. Results： The retention time of PQQ standard was about 7.8 min and the linear correlation coefficient was above 0.9999 from 0.03125 to 1 mg/mL. The average recovery rate was 98.6%. Conclusion： HPLC was a reliable quantitive method in determination of PQQ with high sensitivity and accuracy.