摘要
目的:探讨骨髓间充质干细胞(MSC)移植治疗对大鼠脑缺血后神经功能及脑组织中IL-10、TNF-α表达的影响。方法:原代分离培养MSC。27只大鼠随机分为3组,各9只:假手术组,仅剪开皮肤;模型组,制作局灶性大脑中动脉闭塞(MCAO)模型,术后24 h尾静脉注射PBS;MSC组,制作MCAO模型,术后24 h尾静脉注射移植MSC。术后第3天行改良大鼠神经功能缺损评分(m NSS)评定大鼠神经功能;行为学测试后取梗死灶周围缺血半暗带脑组织,western blot法检测肿瘤坏死因子(TNF)-α、白细胞介素(IL)-10蛋白表达水平。结果:与模型组比较,MSC组m NSS评分升高(P<0.05),脑组织蛋白IL-10表达水平上调(P<0.05),TNF-α表达水平下调(P<0.05)。结论:尾静脉移植MSC治疗可改善局灶性脑缺血大鼠神经功能,可能与移植MSC后脑组织中TNF-α和IL-10水平有关。
Objective： To investigate the effects of transplantation of bone marrow mesenchymal stem cells（MSCs） on the neurological function and the expression of tumor necrosis factor-alpha（TNF-α） and interleukin-10（IL-10） in rats after cerebral ischemia. Methods： Twenty-seven SD rats were randomly divided into 3 groups, sham group, ischemia group and MSC group（n=9 respectively）. The middle cerebral artery occlusion（MCAO） model was induced with filament occlusion method. Rats in the ischemia group were transplanted with primarily cultured MSCs 24 h after MCAO. Neural function was evaluated and the expression of TNF-α and IL-10 were assayed at day 3 after operation. Results： When compared with those in the ischemia group, the neural function was significantly improved（P〈0.05）, the level of TNF-α protein was significantly decreased（P〈0.05） and the level of IL-10 protein was significantly increased（P〈0.05） of those in MSC group. Conclusion： Transplantation of MSCs could promote the nerve function recovery after cerebral ischemia.
出处
《神经损伤与功能重建》
2015年第2期107-109,共3页
Neural Injury and Functional Reconstruction
基金
湖南省教育厅科学研究一般项目(No.13C828)
衡阳市科技局基础研究计划项目(No.2013KJ60)
