钛表面TiO_2纳米管仿生修饰对成骨细胞骨功能基因表达的影响

Effects of Biomimetic Nanotubes on Osteoblast Bone Gene Expression.

目的:钛种植体表面制备TiO2纳米管并在其表面仿生构建钙磷涂层,探索该涂层对成骨细胞骨功能基因表达的影响。方法:采用阳极氧化法在钛基底表面制备TiO2纳米管,并进行碱热处理后在其表面构建仿生钙磷涂层,扫描电镜观察其表面结构的改变,能谱分析其表面化学成分的改变,采用实时定量PCR检测成骨细胞在其表面骨相关基因表达差异。结果:仿生处理可以在TiO2纳米管表面制备出纳米絮状的钙磷涂层,该涂层可以提高碱性磷酸酶和骨钙蛋白基因的表达(P<0.05)。结论:TiO2纳米管仿生修饰更有利于成骨细胞的碱性磷酸酶和骨钙蛋白的基因表达。

Objective：To investigate preparation parameters of TiO2 nanotube layer by biomimetic modification,and to evaluate the osteoblast bone gene expression in vitro.Methods：TiO2nanotube layer was grown using anodization method.TiO2 nanotube layer was modified by biomimetic preparation.SEM and EDS experiment methods were employed to characterize the samples.Osteoblasts were cultivated on the surfaces,and the growth of osteoblasts was then evaluated and analyzed.Results：The Ti implant with nano-Ca-P was acquired by SEM and EDS characterization.The result of real-time PCR showed that the biomimetic nanotubes could advance the ALP and OCN gene expression（P〈0.05）.Conclusion：The nanotubes with nano-Ca-P can express higher bone gene level comprared with nanotubes.