微流控芯片技术在循环肿瘤细胞分离中的研究进展

Advances in Isolating Circulating Tumor Cells With Microfluidic Chips

循环肿瘤细胞(circulating tumor cells,CTCs)是指从原发肿瘤或转移灶脱落、发生上皮-间质转化进入患者外周血血液循环的恶性肿瘤细胞.CTCs在肿瘤研究和临床诊断上的作用逐渐得到认可,外周血中CTCs存在与否以及数量多少不但可以用于肿瘤的早期诊断,还可以用于评估肿瘤预后、监测肿瘤的转移和复发.微流控芯片作为一个高通量、小型化的细胞实验平台,已被应用于CTCs的分选当中.本文综述了用于CTCs捕获的微流控芯片系统的最新研究进展,着重介绍各类芯片的捕获原理、芯片结构和捕获效率,最后对微流控芯片技术在CTCs分选中的应用前景进行了展望.

Circulating tumor ceils （CTCs） are shed by primary or metastatic tumors, undergo the epithelial-mesenchymal transition, and enter into the peripheral blood of patients with metastatic cancers. Its critical roles in cancer research and clinical diagnostics are gradually being recognized. The presence of CTCs, especially the number in peripheral blood can not only be used for early diagnosis of cancer but also be used to assess prognosis, and monitor tumor metastasis and recurrence. Microfluidic chip as a high-throughput, miniaturized cell experimental platform has been used in CTCs sorting. In this review, we highlight recent progresses of CTCs capturing of microfluidic chip system and focus on the capture theory, chip structure and capture efficiency of various types of chips. At last, we prospect for the application foreground of microfluidic chip technology in CTCs isolation.