Ca（OH）2溶液预处理对聚酰胺-胺树枝状大分子诱导脱矿牙本质再矿化的影响

Effect of Ca（OH）2 pre-treated on remineralization of the demineralized dentin induced by polyamidoamine dendrime

目的 评价氢氧化钙[Ca（OH）2]溶液预处理对羧基改性的聚酰胺-胺型树枝状大分子（polyamidoamine dendrime,PAMAM）在脱矿牙本质再矿化中的影响,为治疗牙本质早期龋提供依据.方法 制备32个脱矿人牙本质样本并分为4个组（每组8个）：空白对照组未经任何处理;Ca（OH）2组使用Ca（OH）2溶液进行预处理;PAMAM组使用羧基改性的PAMAM溶液处理;PAMAM＋Ca（OH）2组用羧基改性的PAMAM溶液处理后采用Ca（OH）2溶液预处理.之后各组牙本质样本在人工唾液中矿化2周.利用扫描电镜、能谱分析、X衍射分析评估4组牙本质样本再矿化效果.结果 扫描电镜示空白对照组牙本质小管内无新生物,Ca（OH）2组牙本质小管有少量新生物,PAMAM组部分牙本质小管内被新生物封闭,PAMAM＋ Ca（OH）2组再矿化程度最佳,几乎所有牙本质小管均被再矿化物封闭并呈半球形突出于表面.能谱分析和X射线衍射检测证实矿化物为类羟基磷灰石晶体.结论 羧基改性的PAMAM协同Ca（OH）2溶液预处理可促进脱矿牙本质的再矿化,在治疗牙本质早期龋方面有良好的应用前景.

Objective To evaluate the remineralization effect of Ca（OH）2,pre-treated with carboxylmodified polyamidoamine dendrimer（PAMAM） on demineralized dentin.Methods Thirty-two demineralized dentin models were divided into four groups randomly as follows：control group with no treatment,Ca（OH）2 group pretreated with Ca（OH）2 solution,PAMAM group processed with carboxyl-modified PAMAM,and PAMAM ＋ Ca（OH） 2 group processed with carboxyl-modified PAMAM and pretreated with Ca（OH）2 solution.All samples were immersed in artificial saliva for two weeks.The remineralization effects of dentin discs were evaluated by scanning electron microscope（SEM）,energy dispersive spectrometer（EDS） and X-ray diffraction（XRD）.Results Observed by the SEM,it was showed that in PAMAM ＋ Ca（OH）2 group almost all the dentinal tubules were occluded by the minerals,however this was not found in other groups.The minerals proved were hydroxyapatite through EDS and XRD tests.Conclusions There was potential superiority of the carboxyl-modified PAMAM with Ca（OH）2 solution in promoting the remineralization of initial dentin lesions.