子宫颈癌中Plk1和Cyclin B1、p21^(WAF1)的表达及其临床意义

Expression of Plk1,Cyclin B1 and p21^(WAF1) in cervical carcinoma and their clinical significance

目的探讨Plk1(polo-like kinase1)和Cyclin B1、p21WAF1在子宫颈癌中的表达及其与临床病理因素之间的关系。方法利用组织芯片技术,结合免疫组化Eli Vision法对102例子宫颈癌、20例子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)、20例正常子宫颈组织中Plk1和Cyclin B1、p21WAF1的表达进行检测,并分析相关数据。结果 Plk1在子宫颈癌、CIN中的阳性率分别为70.5%、55.0%,明显高于正常子宫颈组织(10%),差异有统计学意义(P<0.01)。Cyclin B1在子宫颈癌、CIN中的阳性率分别为52.9%,30.0%,明显高于正常子宫颈组织(10.0%),差异有统计学意义(P<0.01)。p21WAF1在子宫颈癌、CIN中的阳性率分别为23.5%、10.0%,明显高于正常子宫颈组织(0),差异有统计学意义(P<0.05)。Plk1、Cyclin B1和p21WAF1在子宫颈癌、CIN中的表达差异均无统计学意义(P>0.05)。Plk1表达与子宫颈癌间质浸润深度相关(P<0.05)。Cyclin B1表达与子宫颈癌间质浸润深度及淋巴结转移相关(P<0.05)。p21WAF1在子宫颈癌中的表达与组织学分级相关(P<0.01)。组织学分级低的子宫颈癌中p21WAF1阳性率高。Plk1和Cyclin B1在子宫颈癌中的表达呈正相关(rs=0.297,P=0.002)。Plk1和p21WAF1在子宫颈癌中的表达呈负相关(rs=-0.403,P<0.001)。结论 Plk1、Cyclin B1和p21WAF1的表达与子宫颈癌的发生、发展相关,且前两者与子宫颈癌预后相关,三者联合检测有可能成为子宫颈癌临床治疗的新靶点。

Abstract： Purpose To investigate the expression of polo-like kinase 1 （Plkl） and Cyclin B1, p21^WAF1 in cervical carcinoma, and to determine the relationship between the expression of the three proteins and tumor clinicopathological features. Methods The expres- sion of Plkl, Cyclin B1 and p21^WAF1 was detected in 102 cases of cervical carcinoma, 20 cases of （cervical intraepithelial neoplasia, CIN）, and 20 cases of nomal cervical tissues by the technique of tissue chip and immunohistochemical staining of EliVision. Statistical analyses of the data were performed with SPSS 19. 0 software. Results The positive rates of Plkl in cervical carcinoma and CIN were 70. 5%, 55.0%, respectively, which were significantly higher than normal cervical tissues （ 10% ） （P 〈0.01 ） ; The positive rates of Cyclin BI in cervical carcinoma and CIN were 52. 9% and 30. 0%, respectively, which were significantly higher than normal cervical tissues （ 10% ） （ P 〈 0. 01 ） ; The positive rates of p21^WAF1 in cervical carcinoma and CIN were 23.5% and 10. 0%, respectively, which were significantly higher than normal cervical tissues （ 0 ） （ P 〈 0, 01 ）. There were no significant differences between cervical carcinoma and CIN in the positive rates of Plkl, Cyclin B1 and p21^WAF1. The expression of Plkl was associated with the depth of carci- noma invasion （P 〈 0. 05 ）, that of Cyclin B1 was associated with lymph node metastases and the depth of carcinoma invasion （P 〈 0.05 ）and that of p21^WAF1 in cervical carcinoma was associated with histological grade （P 〈0. 05 ）. In cervical carcinoma, the expres- sion of Plkl was positively correlated with Cyclin B1 （ rs = 0. 297, P = 0. 002） and negatively correlated with p21^WAF1 （ ra = - 0. 403, P 〈0. 001 ）. Conclusion The expression of Plkl, Cyclin B1 and p21^WAF1 is involved in the occurrence and development of cervical carcinoma, and the former two are also related with prognosis of cervical carcinoma. The combination of the three would provide a new target for clinical treatment.