子宫颈尖锐湿疣组织中HPV感染基因型别分布

Distribution of HPV infective genotypes in condyloma acuminatum tissues of cervix

目的探讨子宫颈尖锐湿疣(condyloma acuminatum,CA)组织中人乳头瘤病毒(human papillomavirus,HPV)感染的基因型别分布及临床意义。方法采用PCR和基因芯片技术对120例子宫颈CA组织进行23种HPV基因分型检测,并对患者的临床病理资料进行分析。结果 120例子宫颈CA组织中检出HPV阳性感染115例,HPV感染率为95.83%(115/120)。其中一重HPV感染者85例,感染率为70.83%(85/120),多重HPV感染者30例,感染率为25.00%(30/120)。一重HPV感染中HPV11型阳性检出率为45.00%(54/120),是最主要的感染型别;其次是HPV6型(27例),阳性检出率为22.50%(27/120)。多重HPV感染中,HPV6+11型6例,占多重感染的20.00%(6/30),是多重感染的主要型别;其次是HPV11+16型(3例),占多重感染的10.00%(3/30)。结论 HPV6型、11型、6+11型、11+16型感染是子宫颈CA的主要致病类型。基因扩增结合基因芯片技术是一种比较适合临床进行HPV分型检测的诊断方法,其敏感性高,特异性好。对子宫颈CA及子宫颈癌/瘤的防治及其疫苗的研究具有十分重要意义。

Purpose To compare the distribution of 23 kinds of human papillomavirus （HPV） genotypes in tissues of condyloma acu- minata （CA） of cervix in 120 women and its clinical significance. Methods Polymerase chain reaction （PCR） and gene-chips tech- nology were utilized for the detection of 23 kinds of HPV genotypes in tissue specimens from 120 cases of CA in cervix and related ma- terials of all subjects were conducted and analyzed. Results There were 115 positive cases in 120 women with CA in cervix and the rate of total HPV infection was 95.83% （115/120）. The rate of single type was 70. 83% （85/120） and multiple types was 25.00% （30/120）. The predominant type of single infection was HPVll and the infective rate was 45.00% （54/120）, followed by HPV6 （22. 50%, 27/120）. Otherwise, the predominant type of multiple infections was HPV6 ＋ 11 with the infective rate of 20. 00% （6/ 30）, and HPV11 ＋ 16 infection accounted for 10. 00% （3/30）. Conclusions HPV11,6, 6 ＋ 11 and 11 ＋ 16 are the main genotypes in the pathogenesis of CA in cervix in 120 women. PCR and gene-chip technology can detect single and multiple HPV genotyping in tis- sues of CA in cervix with high sensitivity and specificity. Detection of HPV genotypes could be used to understand the prevalence situa- tion of HPV infection in tissues of CA and tumors of cervix and further to provide references for the research and development of HPV vaccine in women