中药单体白花丹醌对鲍曼不动杆菌的耐药逆转作用

Drug resistance reversing effect of Chinese herb monomer plumbagin on baumanii

目的:探索中药单体白花丹醌对临床分离的鲍曼不动杆菌耐药性的逆转作用。方法:取临床分离的鲍曼不动杆菌53株,检测其对临床常用的14种抗菌药物的敏感性。观察中药单体白花丹醌对标准菌株的直接作用。在确定白花丹醌以及羰基氰化氯苯腙(CCCP)的工作浓度以后,观察对比白花丹醌与阳性药CCCP的外排抑制试验结果。结果:临床分离的53株鲍曼不动杆菌对临床常用的14种抗菌药物耐药率较高,其中氨苄西林、头孢唑啉、头孢呋辛、头孢西丁耐药率最高,分别为100%、100%、98.1%、98.1%。其余药物耐药率也均在50%以上。白花丹醌对标准质控菌的直接抗菌作用较弱。而白花丹醌与CCCP均显示了对庆大霉素、诺氟沙星、米诺环素和氯霉素的增敏作用,其外排阳性率分别达11.3%和18.9%,3.8%和7.5%,13.2%和17.0%,28.3%和20.8%,两组之间无统计学差异(P>0.05)。结论:鲍曼不动杆菌临床耐药情况严重,而白花丹醌作为一种中药单体可能具有一定的耐药逆转作用。

AIM：To explore the drug resistance reversing effect of Chinese herb simple substance plumbagin on baumanii.METHODS：Fifty-three strains of baumanii were isolated from patients.The antibacterial activities of 14 kinds of antibacterial drugs commonly used in clinic were detected.The direct antibacterial activities of Chinese herb monomer of plumbagin on standard strains were studied.After the determination of plumbagin and carbonyl cyanide mchlorophenylhydrazone（CCCP）work concentration,the efflux inhibition test results of plumbagin and CCCP were compared.RESULTS：The resistance rate of 53 clinical isolates of baumanii to 14 kinds of clinical commonly used antimicrobial drugs was high.The ampicillin,cefazolin,cefuroxime,cefoxitin resistance rate was highest,respectively 100%,100%,98.1% and98.1%.The rest of the drug-resistant rate is above 50%.The direct antimicrobial effect of plumbagin on quality standard bacteria was weak.Plumbagin and CCCP showed increasing sensitization to gentamicin,norfloxacin,minocycline hydrochloride and chloramphenicol.Their positive rates of efflux were 11.3% and18.9%,3.8% and 7.5%,13.2% and 20.8%,28.3% and 20.8%,respectively.There were no statistical differences between two groups.CONCLUSION：The clinical drug resistance of baumanii is in serious condition,and plumbagin as a kind of traditional Chinese medicine monomer may have a certain role in the drug resistance reversal.