氧化苦参碱对高糖毒性下血管内皮细胞的保护作用

Protective effect of oxymatrine on vein endothelial cell from glucose toxicity

目的研究氧化苦参碱对A2B受体介导高糖毒性下人脐带静脉血管内皮细胞(HUVEC)保护作用。方法采用传代细胞系培养方法培养人脐静脉血管内皮细胞,细胞分组如下:5.5 mmol·L-1对照糖组(Control),22.2 mmol·L-1高糖组(22.2 mmol·L-1),44.4 mmol·L-1高糖组(44.4 mmol·L-1);对照糖+氧化苦参碱组(control+OMT),22.2mmol·L-1高糖+氧化苦参碱组(22.2 mmol·L-1+OMT),44.4 mmol·L-1高糖+氧化苦参碱组(44.4 mmol·L-1+OMT)。采用MTS法观察高糖和氧化苦参碱保护效应;结合蛋白印迹、RT-PCR等方法观察HUVEC中A2B受体的表达变化。结果高糖培养可引起HUVEC受损和活性下降。氧化苦参碱(OMT)可明显减少高糖性HUVEC损伤和活性下降,并对A2B的表达有明显抑制作用。结论氧化苦参碱对高糖条件下的HUVEC有保护作用,此作用可能通过抑制A2B的表达而实现。

Aim To study the effect of oxymatrine（ OMT） on protecting human umbilical vein endothelial cells（ HUVECs） from toxicity induced by high glucose in vitro. Methods The HUVECs were cultured with medium containing different concentrations of glucose or OMT. The cells were randomly divided into 6groups： 5. 5 mmol·L^-1control group（ Control）,22. 2mmol·L^-1high glucose（ 22. 2 mmol · L^- 1）,44. 4mmol·L^- 1high glucose（ 44. 4 mmol·L- 1）,control＋ OMT（ control ＋ OMT）,22. 2 mmol·L^- 1high glucose ＋ OMT（ 22. 2 mmol·L^- 1＋ OMT）,44. 4 mmol·L^-11high glucose ＋ OMT（ 44. 4 mmol · L^- 1＋OMT）. The protective effect of oxymatrine was assessed by MTS assays. The expression of A2 Bin HUVECs was detected by Real-time quantitative PCR（ q PCR） and Western Blot methods. Results The glucose was shown to have caused cytotoxicity in HUVECs. Oxymatrine（ 3 μmol·L- 1） was found to have protected HUVECs from glucose toxicity effectively,and reduced the expression of A2 Bsignificantly. Conclusion Oxymatrine can obviously protect HUVECs from cytotoxicity induced by high glucose and the effect is performed partly by decreasing A2 Bexpression.