摘要
目的:探讨增龄和心房颤动(房颤)所致的左心房结构重构是否与miRNAs的表达改变相关。方法由新疆医科大学第一附属医院动物实验中心提供的21只犬分为3组:成年窦性心律组、老年窦性心律组和老年持续性房颤组。通过持续快速心房起搏建立房颤模型,用实时荧光定量聚合酶链反应( qRT-PCR)的方法检测3组犬左心房心肌细胞miRNA-1、miRNA-21、miRNA-29和miRNA-133的表达变化;应用光镜、电镜检测各组左心房心肌病理组织学和超微结构的改变。 DNA断裂的原位末端标记法( TUNEL)检测细胞凋亡指数。结果在窦性心律时,与成年组相比较,老年组左心房心肌细胞miRNA-21,miRNA-29的表达水平呈现显著上调趋势(miRNA-21,2.3671±0.3056对1.3529±0.1921;miRNA-29,2.1929±0.2726对1.2430±0.1828,P〈0.05);miRNA-1,miRNA-133的表达水平呈现出显著下调趋势(miRNA-1,0.7518±0.1009对1.2036±0.1328;miRNA-133,1.0438±0.1282对1.2705±0.2025, P〈0.05);与窦性心律老年组相比较,持续性房颤老年组左心房心肌细胞miRNA-1、miRNA-21、miRNA-29的表达水平显现出显著上调趋势( miRNA-1,1.2475±0.2091对0.7518±0.1009;miRNA-21,3.8251±0.316对2.367±0.3056;miRNA-29;3.4532±0.3076对2.1929±0.2726,P〈0.05);miRNA-133的表达水平显现显著下调趋势(miRNA-133,0.7439+0.1026对1.0438+0.1292,P〈0.05)。伴随增龄与房颤,犬心肌纤维化程度、细胞超微结构及凋亡指数差异有统计学意义(P〈0.05)。结论伴随增龄与房颤而显现的miRNA-1、miRNA-21、miRNA-29和miRNA-133的表达改变与病理性心房重构相关。
Objective The study was designed to decipher whether the structure remodeling of left at-rial induced by aging and atrial fibrillation ( AF ) correlated with changes in miRNAs expres-sion. Methods Dogs were divided into 3 groups randomly:adult and old groups in sinus rhythm ( SR) and old group with chronic AF. AF model was induced by rapid atrial pacing. Expressions of miRNA-1,miRNA-21, miRNA-29 and miRNA-133 were measured by Quantitative Real-Time Polymerase Chain Reaction ( qRT-PCR) . Pathohistological and ultrastructural changes were analyzed by light and electron microscopy. Apoptosis index of myocytes was detected by TdT-mediated dUTP nick end labeling( TUNEL) . Results In sinus rhythm group,compared to the adult group,the expressions of miRNA-21,29 were significantly increased( miRNA-21, 2. 3671±0. 3056 vs. 1. 3529±0. 1921;miRNA-29,2. 1929±0. 2726 vs. 1. 2430±0. 1828,P〈0. 05),whereas the expressions of miRNA-1,miRNA-133 showed obviously down-regulation tendencye in the aged group(miRNA-1, 0. 7518±0. 1009 vs. 1. 2036±0. 1328;miRNA-133,1. 0438±0. 1282 vs. 1. 2705±0. 2025,P〈0. 05). Compared to the aged group in sinus rhythm,the expressions of miRNA-1,miRNA-21,miRNA-29 were significantly in-creased in the old group in AF(miRNA-1,1. 2475±0. 2091 vs. 0. 7518±0. 1009;miRNA-21,3. 8251±0. 316 vs. 2. 3671±0. 3056;miRNA-29;3. 4532±0. 3076 vs. 2. 1929±0. 2726,P〈0. 05). In contrast,the expressions of miRNA-133 showed obviously down-regulation tendency(miRNA-133,0. 7439+0. 1026 vs. 1. 0438+0. 1292,P〈0. 05). Samples of atrial tissue showed statistical significance changes(all P〈0. 05)in fibrosis degree,ultra-structural and apoptosis index with aging and/or in AF dogs. Conclusion These changes in miRNAs expres-sion may be responsible for pathological atrialremodeling with aging and AF.
出处
《中华心律失常学杂志》
2015年第1期60-64,共5页
Chinese Journal of Cardiac Arrhythmias
基金
新疆维吾尔自治区自然科学基金
关键词
心房颤动
增龄
结构重构
miRNAs
Atrial fibrillation
Aging
Structuralremodelling
miRNAs