摘要
CHR729是水稻(Oryza sativa L.)CHD3类染色质重塑因子,广泛参与水稻生长发育进程,并影响全基因组明显表达和组蛋白修饰,测定了多种植物内源激素在chr729突变体中的含量,探讨了CHR729与激素代谢之间的联系。结果表明,在苗期地上部分和成熟期剑叶中,chr729突变体内源生长素(IAA),脱落酸(ABA),茉莉酸(JA)含量明显降低,生长素合成以及ABA合成基因的表达在chr729突变体中均呈下调表达。树脂切片结果显示,chr729突变体中茎秆成熟组织细胞明显变小,居间分生组织细胞大小无明显变化。苗期检测细胞分裂素氧化酶/脱氢酶(CKX)基因表达发现,chr729突变体中CKX1、CKX2、CKX4基因表达量升高,伴随一些位点组蛋白变体H2A.Z富集程度的上升,表明CHR729可能抑制H2A.Z在基因组上的装载,广泛参与水稻激素代谢调控。
CHR729 is a CHD3 chromatin remodeler playing multiple roles in growth and development of rice. Previous studies indicated that CHR729 modulate a subset of gene expression and histone modifications. The contents of multiple endogenous phytohormones were measured. The correlation of CHR729 with hormone dynamics was investigated. A significant decrease in endogenous auxin, abscisic acid and jasmonic acid was observed in chr729 seedlings and mature flag leaves. Genes involved in auxin or abscisic acid biosynthesis were down-regulated in chr729 mutant. The cell length in mature tissue and intercalary meristem in stem was examined. A significant decrease in cell length of mature tissue of chr729 was found. Cell size of intervening meristem had no significant changes. Some cytokinin oxidase / dehydrogenase genes were up-regulated in chr729 mutant, with a relatively high enrichment of histone variant H2 A.Z at some loci, indicating the involvement of CHR729 in the deposition / removal of H2 A.Z. It is implied that CHR729 was involved in multiple hormone metabolisms of rice.
出处
《湖北农业科学》
2015年第2期468-473,共6页
Hubei Agricultural Sciences
