扶正散结汤拆方对Lewis肺癌小鼠血清血管内皮生长因子、血管抑素及内皮抑素的影响

Study on the effect of separated prescriptions Fuzheng Sanjie Recipe on Lewis lung cancer mouse serum VEGF, AS and ES

目的探讨通过检测血清相关细胞因子分析扶正散结方抑制肿瘤生长在微血管生成方面作用机制的可行性。方法 C57BL/6小鼠80只,随机分成空白组、模型组、顺铂组、扶正组、化痰组、活血组、解毒组和联合用药组,每组10只,造模后分别给与相应药物干预,连续14天,同时称取体质量及测量肿瘤长短径,于第14天取血后留取血清,以ELISA法检测小鼠血清血管内皮生长因子(vascular endothelial growth factor,VEGF)、血管抑素(angiostatin,AS)、内皮抑素(endostatin,ES)的表达。结果肿瘤生长速度以顺珀与联用组最慢,其余中药组次之,模型组生长最快。VEGF含量,以联用组含量最低,其次为活血组与顺珀组,其余中药组含量较高(P<0.01)。AS含量,各组均较空白组降低(P<0.05),模型组尤为显著(P<0.01),以顺珀组及联用组降低程度最轻。各组ES含量较空白组均明显降低(P<0.01),以模型组最著,联用组及扶正组与DDP组最接近(P>0.05),化痰组、活血组和解毒组ES含量则较低(P<0.01)。结论扶正散结汤各组拆方中药均能抑制肿瘤生长,其作用机制可能与抑制VEGF、上调AS和ES的水平从而达到抑制血管生成有关;血清学检测可以作为研究中药在肿瘤微血管生成方面作用机制的辅助方法。

Objective To investigate the feasibility analysis of Fuzheng Sanjie Recipe angiogenesis in tumor growth in terms of the mechanism of inhibition by serum cytokines. Methods 80 C57 BL /6mice were randomly divided into controlgroup,Model group,DDP group,Fuzheng group,Huatan group,Huoxue group,Jiedu group and Combination group. After establishing the model of Lewis Lung Cancer,give them different drugs intervention for 14 days and thenmeasured the diameter of tumor and the mice weight.ELISA was used to detect the changes of peripheral serum VEGF,AS and ES in the mice. Results Tumor growth in combination group and DDP group were slow,followed by the rest TCM groups,the tumor growth in model group was the fastest. the content of VEGF levels in the combination group was the lowest,followed by Huoxue group and DDP group,the rest of the TCM group content was higher（ P〈0. 01）. AS content,compared with the control group,other groups were decreased（ P〈0. 05）,model group was particularly significant（ P〈0. 01）,with DDP group and combination group decreased less. ES content,compared with the control group,the other group were significantly lower（ P〈0. 01）,the model group decreased most,and there was no difference between the combination group,Fuzheng group and DDP group,the ES content in Huatan group,Huoxue group and Jiedu group were lower（ P〈0. 01）. Conclusion Separated Fuzheng Sanjie recipe can all inhibit the growth of tumors,and its mechanism may be related to inhibiting the expression of VEGF and upregulation the content of AS and ES; the results of serological testcan be used as an auxiliary method of TCM in terms of tumor angiogenesis mechanisms.