希望I号液对大鼠吸入臭氧肺损伤的保护作用研究

The protection effect of Hope I Decoction on ozone inhalation-induced injury in rats

目的观察希望I号液对大鼠吸入臭氧导致肺损伤的保护作用。方法 48只雄性SD大鼠随机分为6组,即对照组、模型组、维生素E组(0.0135 g/kg)、希望I号液低、中、高剂量组(0.045、0.090、0.180g/kg),除对照组其余各组暴露臭氧(1.2±0.2 ppm)10 h/d×45 d。维生素E组、希望I号液低、中、高剂量组在臭氧造模同时给药。检测血清中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化脂质(lipid peroxide,LPO)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)含量,HE、Masson染色观察肺组织变化,免疫组化方法观察肺组织中肺表面活性蛋白A含量。结果与模型组相比,希望I号液各组SOD、CAT、GSH-Px活性增加,LPO水平降低;希望I号液各给药组炎性细胞渗出胶原纤维含量减少;希望I号液各组肺表面活性蛋白A含量明显高于模型组。结论希望I号液对大鼠吸入臭氧导致损伤具有良好的保护作用。

Objective To evaluate the protection effect of Hope I decoction on ozone inhalationinduced injury in rats. Methods 48 male SD rats were randomly divided into 6 groups,i. e.,control,model,VE（ 0. 0135 g / kg）,Hope I decoction at three dosages（ 0. 045,0. 090,0. 180 g / kg respectively）.Modeling rats were exposed to ozone in an exposure cabinet（ 10 h / d × 45d）,then some of them were intragastrically administrated with vitamin E or Hope I decoction. Then,the level of SOD,LPO,CAT and GSHPx in rat serum were detected and pathologic change of lung tissue were observed,Immunohistochemistry for surfactant protein A（ SP-A） content were observed. Results The activity of SOD,CAT and GSH-Px in serum were increased and LPO level were reduced in all Hope I groups. Histopathological slides of these three groups also showed an attenuated extent of alveoli dilatation and collapse induced by ozone exposure,surfactant protein A of these three groups were significantly enhanced. Conclusion Hope I decoction hasgreat protection effect on ozone inhalation-induced injury in rats.