晚期胃肠道肿瘤正虚程度与外周血肿瘤缺氧相关蛋白关系

Relationship of healthy Qi deficiency and serum hypoxia-related proteins in patients with advanced gastrointestinal cancer

目的探讨晚期胃肠道肿瘤正虚程度与外周血肿瘤缺氧相关蛋白之间的关系。方法普陀医院肿瘤科2012年4月1日到2013年4月10日IV期胃肠道肿瘤患者46例,以卡氏(Karnofsky Performance Status,KPS)评分、中医症候(气短、纳差、爪甲不荣)作为评价患者正虚指标,并根据正虚指标分别分组,采用酶联免疫吸附法检测患者血清中血管内皮生长因子(vascular endothelial growth factor,VEGF)、骨桥蛋白(osteopontin,OPN)、碳酸酐酶IX(carbonic anhydrase IX,CAIX)水平。结果经成组t检验,晚期胃肠道肿瘤患者KPS≤60分组与KPS>60分组比较,前者VEGF、OPN、CAIX水平分别是后者的1.75倍(P<0.05)、1.10倍(P>0.05)、1.68倍(P<0.05)。晚期胃肠道肿瘤具有气短、纳差、爪甲不荣症状患者外周血VEGF、OPN、CAIX水平均明显高于无上述症状患者(爪甲不荣组CAIX除外),其中以VEGF指标均有统计学意义(P<0.05)。结论晚期胃肠道肿瘤正虚程度与外周血缺氧相关蛋白VEGF、OPN、CAIX相关。

Objective To investigate the relationship between the extent of healthy Qi deficiency and hypoxia-related proteins in the advanced gastrointestinal cancer patients. Methods We recruited 46 patients with stage IV advanced gastrointestinal cancer between April 1, 2012 and April 10, 2013. The KPS score and TCM symptoms （ shortness of breath, poor appetite, matt fingernail） for evaluating patients were determined for evaluatingpatients with healthy Qi deficiency and setting groups ELISA test were performed to examine the expression level of vascular endothelial growth factor（VEGF）, osteopontin（OPN） and carbonic anhydrase IX（CAIX）. Results After a group t test, compared advanced gastrointestinal cancer patients KPS≤60 group with KPS〉60 group, the former of VEGF, OPN, CAIX respectively was 1. 75 times（P〈0. 05）,1. 10 times（P〉0. 05）,1. 68 （P〈0. 05）that of the latter. Advanced gastrointesti-nal cancer have the symptoms of shortness of breath, poor appetite, matt fingernail in patients with VEGF, OPN, CAIX levels were significantly higher than those without these symptoms （ except CAIX in matt fin-gernail group）, which VEGF were statistically significant（P〈0. 05）. Conclusion The expression of hypoxia-related proteins increased gradually with the healthy Qi deficiency degree in the advanced gastrointestinal cancer patients.