姜黄素对佐剂性关节炎大鼠血清骨保护素和核因子κB受体活化因子配体蛋白表达及骨密度的影响

Effect of curcumin on the expression of blood serum osteoprotegerin and receptor activator of nuclear factor-κB ligand protein and bone mineral density in adjuvant arthritis rats

目的观察姜黄素对佐剂性关节炎(adjuvant arthritis,AA)大鼠血清骨保护素(osteoprotegerin,OPG)、核因子κB受体活化因子配体(receptor activator for nuclear factor-κB ligand,RANKL)表达以及骨密度的影响,探讨姜黄素防止类风湿关节炎(rhuematoid arthritis,RA)骨破坏的作用机制。方法取SD大鼠30只,随机分为模型组、姜黄素组及正常组,第28天腹主动脉取血,采用ELISA法来检测血清中RANKL、OPG蛋白的表达,取血后处死大鼠,剥离大鼠右侧胫骨,行骨密度检查。结果 (1)与正常组比较,造模组即模型组与姜黄素组大鼠血清RANKL均显著升高(P<0.01),血清OPG显著降低(P<0.01),RANKL/OPG比值显著升高(P<0.01);(2)与模型组比较,治疗组即姜黄素组血清RANKL显著降低(P<0.01),血清OPG均显著升高(P<0.01),RANKL/OPG比值均显著降低(P<0.01);(3)3组大鼠胫骨整体骨密度无显著性差异(P>0.05),姜黄素组大鼠兴趣区骨密度值显著高于模型组(P<0.01)。结论姜黄素对AA大鼠具有良好的治疗作用,能够通过调节血清RANKL及OPG的表达,来调节OPG/RANKL通路,从而提高AA大鼠骨密度,抑制其骨丢失。

Objective By observing the effects of curcumin on blood serum osteoprotegerin （OPG） and receptor activator of nuclear factor-κB ligand （RANKL） protein and bone mineral density of adjuvant arthritis （ AA） rat models, we try to explore the possible mechanism of curcumin preventing bone destruction in rheumatoid arthritis. Methods 30 SD rats were randomly divided into model group, curcu-min group and normal group. On day 28 draw blood from abdominal aorta to detect the expression of blood serum RANKL and OPG protein by ELISA method. After drawing blood, rats were sacrificed and stripped the right tibia, to examine bone density. Results （ 1 ） Compared with the normal group, the serum RANKL of model group and curcumin group rats were significantly increased （P〈 0. 01）, the serum OPG expression were significantly decreased （P〈 0. 01）, the ratio of RANKL/OPG were significantly increased （ P〈 0. 01）;（2） Compared with the model group, the serum RANKL of curcumin group was significantly decreased （P〈 0. 01）, the serum OPG was significantly increased （P〈 0. 01）, the ratio of RANKL/OPG was significantly decreased （P〈 0. 01）;（3） There are no significant differences among the 3 groups’ tibia bone mineral density （P〉 0. 05）. The bone mineral density in region of interest of curcumin group＇ was significantly higher than that of the model group （ P〈 0. 01 ） . Conclusions Curcumin has a good therapeutic effect on AA rats. Curcumin regulates the expression of blood serum RANKL and OPG, thus regulate the OPG/RANKL pathway, thereby improve bone mineral density in AA rats to inhibit the bone loss.