结核分枝杆菌HspX基因真核表达载体构建

Construction of Eukaryotic Expression Vector of Mycobacterium Tuberculosis Hsp X Gene

为构建结核分枝杆菌Hsp X基因与增强型绿色荧光蛋白(EGFP)融合基因的真核表达载体,文章采用PCR法扩增Hsp X基因,定向插入到质粒p EGFP-N1的多克隆位点。结果显示,融合基因真核表达载体,经双酶切鉴定、基因测序鉴定克隆片段大小和序列正确。文章成功构建了p EGFP-N1-Hsp X融合基因真核表达载体。

In order to construct a recombinant plasmid carrying enhanced green fluorescent protein（EGFP）and Mycobacterium tuberculosis Hsp X gene,Hsp X gene was amplified by PCR and inserted into a multiple cloning site of eukaryotic expression vector p EGFP-N1. The results shows Hsp X eukaryotic expression vector labeled with FLAG tag was successfully constructed as demonstrated by double enzyme digestion and DNA sequencing. The conclusion is that the eukaryotic expression plasmid p EGFP-N1-Hsp X has been constructed successfully.