氧化低密度脂蛋白对体外血管内皮细胞损伤的剂量反应关系

Dose-response relationship of oxidized low density lipoprotein on human umbilical vein vascular endothelial cells damage in vitro

目的:研究氧化低密度脂蛋白(ox-LDL)对体外培养的人脐静脉血管内皮细胞(HUVEC)造成的损伤效应。方法:分别用不同浓度的ox-LDL作用于对数生长期的HUVEC细胞24 h,进行细胞形态学观察比较,采用四唑盐(MTT)比色法测定细胞活力,计算细胞生长抑制率,并测定细胞中丙二醛(MDA)含量。结果:随着ox-LDL浓度的增加,细胞形态发生明显变化,变得结构松散,ox-LDL高浓度时甚至出现细胞破碎、死亡。MTT实验的吸光度值(OD)在ox-LDL浓度为50、100、200μg/m L时,与对照组相比差异均具有统计学意义(P<0.05)。不同浓度干预24 h后细胞中MDA含量与MTT实验结果一致。当ox-LDL浓度为50、100、200μg/m L时,细胞抑制率分别达到23.2%、45.2%、62.0%。结论:当HUVEC细胞暴露于ox-LDL 24 h后,干预浓度越大细胞活力越低,细胞生长抑制率越大,细胞中MDA含量增加。ox-LDL浓度为200μg/m L以内时,ox-LDL对HUVEC细胞可造成较明显的氧化损伤且呈现剂量效应关系。

Objective：To explore the dose-response relationship of oxidized low density lipoprotein（ox-LDL） on human umbilical vein vascular endothelial cells（HUVEC） damage in vitro. Methods： HUVEC were treated with different concentrations of ox-LDL for 24 h.Morphological change was observed. And then cell viability was measured by MTT and growth inhibition ratios were calculated. The content of MDA in endothelium cells was measured. Results： With the increase of the concentration of ox-LDL, cells morphology of HUVEC was deteriorated. Cells became loose, broken and eventually dead. When cells were exposed to different doses of ox-LDL for 24 h, compared with control group, cell viabilities of 50, 100 and 200 μg/m L groups were decreased respectively and the differences were statistically significant（P〈0.05）. The statistical result of MDA concentration in cells after 24 h was consistent with MTT. The growth inhibition ratios for50, 100, 200 μg/m L group were 23.2%, 45.2%, 62.0%. Conclusion： When HUVEC are exposed to ox-LDL for 24 h, the cell viability decreases with the rise of the ox-LDL concentration while the cell growth inhibition rate and MDA content in the cells increase. When the concentration of ox-LDL is below 200 μg/m L, the HUVEC suffer marked oxidation damage and present dose effect relationship.