TSLP与调节性T淋巴细胞在慢性鼻窦炎中的表达

Expression of TSLP and its relationship with the infiltrsting regulatory T cells in chronic rhinosinusitis

目的观察胸腺基质淋巴细胞生成素(TSLP)及CD4+CD25+Foxp3+调节性T细胞(Tregs)在单纯慢性鼻窦炎中的表达,探讨二者在慢性鼻窦炎发生发展过程中可能的作用机制。方法收集25例慢性鼻窦炎患者的鼻窦黏膜为实验组,28例鼻中隔偏曲患者的下鼻甲黏膜为对照组,采用Q-RT-PCR和免疫组化技术检测各组鼻腔黏膜中调节性T细胞、Foxp3 mRNA和TSLP mRNA的表达,并分析TSLP与调节性T细胞、Foxp3 mRNA的相关关系。结果 1TSLP mRNA、Foxp3 mRNA、调节性T细胞在慢性鼻窦炎中的表达明显高于对照组(P<0.05),TSLP与Foxp3 mRNA的表达在慢性鼻窦炎中呈正相关(r=0.977 3,P<0.000 1);2根据免疫组化结果计数Tregs数量,TSLP与调节性T细胞在慢性鼻窦炎中的表达也呈正相关关系(r=0.864 6,P<0.000 1)。结论 TSLP可通过诱导调节性T细胞的发育和增殖,维持免疫自稳,参与慢性鼻窦炎的发生发展过程,其具体的机制有待进一步研究。

Objective To determine the expression of thymic stromal lymphopoietin（TSLP）and regulatory T cells （C D4 ＋C D25 ＋ Foxp3 ＋ Treg）in human chronic rhinosinusitis （CRS ） and to analyze their pathogenesis in CRS. Methods The sinus mucosa from 25 patients with CRS was studied.Inferior nasal turbinate from 28 patients with nasal septum deviation served as the control.Their histological characteristics were analyzed by HE stain.The expressions of TSLP and Foxp3 mRNA were detected by Q-RT-PCR.Immunohistochemistry was used to detect C D4 ＋C D25 ＋Foxp3 ＋Treg cells.The correlation of TSLP with the number of Tregs and the correlation of TSLP with Foxp3 mRNA were evaluated.Results The expressions of TSLP mRNA,Foxp3 mRNA and Treg cells were significant higher in CRS compared with the control group（P 〈0.05）.There was positive correlation between TSLP and Foxp3 mRNA（r =0.977 3, P 〈0.000 1）.At the same time,there was positive correlation between TSLP and the number of Tregs（r =0.864 6,P 〈0.0001）.Conclusion TSLP could play an important role in CRS by inducing Treg cells.