基于Bacillus cereus 3BS1的生物肥研究

Research on Bio-fertilizer Based on the Bacillus cereus 3BS1

[目的]本研究主要目的是探索海藻酸钠细菌颗粒生物肥最佳基材配方,对此种生物肥在贮存及应用中的性质及土壤中降解特性进行研究。[方法]生物肥最佳包埋基材的筛选,细菌计数法研究生物肥在4℃和室温条件下贮存时细菌活性的变化情况,通过生物肥直径变化研究其在土壤中的生物降解情况。[结果]研究表明,基材配方为1.5%海藻酸钠+0.5%大米粉的生物肥,能够达到最佳构形及最高细菌活性,与未加入大米粉的1.5%纯海藻酸盐基材相比,细菌包含率最高,为112.3%,且在贮存过程中能够保持细菌的高活性。此生物肥埋在土壤中3 cm深时降解最快,11 d可完全降解。[结论]获得一种低成本、实用、高效的生物肥,基材最佳配方为1.5%海藻酸钠+0.5%大米粉。

[Objective] The main purpose of this study istoexploretheoptimumformulaofeffective matrixforalginatebacterial particles bio-fertilizer, and to study the biological fertilizer＇s storage and application properties,and the property of degradation in soil. [Method] Screening of biological fertilizer the best embedding substrate, bacteria counting method was employed to study the biological activity of bacteria fertilizer under 4 ℃ and room temperature, to study the biodegradation of bio-fertilizer in soil by the diameter changes.[Results] Bio-fertilizer of 1.5% alginate ＋ 0.5% rice starch had the best formation and highest bacterial activity during storage.The embedding rate of it reached 112.3% compared with only 1.5% alginate.Bio- fertilizer could be complete decomposed in 11 days at the depth of 3 cm in soil. [Conclusion] To successfully optimize a low-cost effective bacteria carrier was successfully optimized, the optimum formula of matrix was 1.5% alginate＋0.5% rice starch.